Information on EC 2.4.1.334 - 1,3-alpha-oligoglucan phosphorylase

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The expected taxonomic range for this enzyme is: Lachnoclostridium phytofermentans

EC NUMBER
COMMENTARY hide
2.4.1.334
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RECOMMENDED NAME
GeneOntology No.
1,3-alpha-oligoglucan phosphorylase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
[(1->3)-alpha-D-glucosyl]n + phosphate = [(1->3)-alpha-D-glucosyl]n-1 + beta-D-glucose 1-phosphate
show the reaction diagram
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
SYSTEMATIC NAME
IUBMB Comments
1,3-alpha-D-glucan:phosphate beta-D-glucosyltransferase
The enzyme, isolated from the bacterium Clostridium phytofermentans, catalyses a reversible reaction. Substrates for the phosphorolytic reaction are alpha-1,3-linked oligoglucans with a polymerisation degree of 3 or more. Nigerose (i.e. 3-O-alpha-D-glucopyranosyl-D-glucopyranose) is not phosphorylyzed but can serve as substrate in the reverse direction (cf. EC 2.4.1.279, nigerose phosphorylase).
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranose 1-phosphate
show the reaction diagram
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranosyl 1-phosphate
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
show the reaction diagram
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranose 1-phosphate
show the reaction diagram
3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranose 1-phosphate
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
show the reaction diagram
kojibiose + beta-D-glucopyranose 1-phosphate
? + phosphate
show the reaction diagram
-
-
-
?
maltose + beta-D-glucopyranose 1-phosphate
? + phosphate
show the reaction diagram
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-
-
?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranose 1-phosphate
show the reaction diagram
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranose 1-phosphate
show the reaction diagram
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.9
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose
pH 7.0, 30C, phosphorolysis reaction, release of beta-D-glucopyranose 1-phosphate
1 - 3.2
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose
3.1
3-O-alpha-D-glucopyranosyl-D-glucopyranose
pH 7.0, 30C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate
8.3
beta-D-glucopyranose 1-phosphate
pH 7.0, 30C, reverse phosphorolysis reaction, acceptor 3-O-alpha-D-glucopyranosyl-D-glucopyranose, i.e. nigerose
50
kojibiose
pH 7.0, 30C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate
3.8
maltose
pH 7.0, 30C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate
2.6 - 11
phosphate
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
8.5
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose
Lachnoclostridium phytofermentans
A9KSL6
pH 7.0, 30C, phosphorolysis reaction, release of beta-D-glucopyranose 1-phosphate
11 - 17
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose
8.7
3-O-alpha-D-glucopyranosyl-D-glucopyranose
Lachnoclostridium phytofermentans
A9KSL6
pH 7.0, 30C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate
5.1
beta-D-glucopyranose 1-phosphate
Lachnoclostridium phytofermentans
A9KSL6
pH 7.0, 30C, reverse phosphorolysis reaction, acceptor 3-O-alpha-D-glucopyranosyl-D-glucopyranose, i.e. nigerose
3.5
kojibiose
Lachnoclostridium phytofermentans
A9KSL6
pH 7.0, 30C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate
2.7
maltose
Lachnoclostridium phytofermentans
A9KSL6
pH 7.0, 30C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.4
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose
Lachnoclostridium phytofermentans
A9KSL6
pH 7.0, 30C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate
194826
2.8
3-O-alpha-D-glucopyranosyl-D-glucopyranose
Lachnoclostridium phytofermentans
A9KSL6
pH 7.0, 30C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate
15208
1.6
beta-D-glucopyranose 1-phosphate
Lachnoclostridium phytofermentans
A9KSL6
pH 7.0, 30C, reverse phosphorolysis reaction, acceptor 3-O-alpha-D-glucopyranosyl-D-glucopyranose, i.e. nigerose
194820
0.069
kojibiose
Lachnoclostridium phytofermentans
A9KSL6
pH 7.0, 30C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate
1470
0.5
maltose
Lachnoclostridium phytofermentans
A9KSL6
pH 7.0, 30C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate
86
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8
phosphorolytic activity
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21