Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.334 extracted from

  • Nihira, T.; Nishimoto, M.; Nakai, H.; Ohtsubo, K.; Kitaoka, M.
    Characterization of two phosphorylases for alpha-1,3-oligoglucans from Clostridium phytofermentans (2014), J. Appl. Glycosci., 61, 59-66.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 Lachnoclostridium phytofermentans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1 2 3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose pH 7.0, 30°C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate Lachnoclostridium phytofermentans
2.6
-
phosphate pH 7.0, 30°C, phosphorolysis reaction of 3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose, release of beta-D-glucopyranose 1-phosphate Lachnoclostridium phytofermentans
2.9
-
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose pH 7.0, 30°C, phosphorolysis reaction, release of beta-D-glucopyranose 1-phosphate Lachnoclostridium phytofermentans
3.1
-
3-O-alpha-D-glucopyranosyl-D-glucopyranose pH 7.0, 30°C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate Lachnoclostridium phytofermentans
3.2
-
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose pH 7.0, 30°C, phosphorolysis reaction, release of beta-D-glucopyranose 1-phosphate Lachnoclostridium phytofermentans
3.8
-
maltose pH 7.0, 30°C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate Lachnoclostridium phytofermentans
8.3
-
beta-D-glucopyranose 1-phosphate pH 7.0, 30°C, reverse phosphorolysis reaction, acceptor 3-O-alpha-D-glucopyranosyl-D-glucopyranose, i.e. nigerose Lachnoclostridium phytofermentans
11
-
phosphate pH 7.0, 30°C, phosphorolysis reaction of 3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose, release of beta-D-glucopyranose 1-phosphate Lachnoclostridium phytofermentans
50
-
kojibiose pH 7.0, 30°C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate Lachnoclostridium phytofermentans

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Lachnoclostridium phytofermentans 5829
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate Lachnoclostridium phytofermentans
-
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranose 1-phosphate
-
r
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate Lachnoclostridium phytofermentans DSM 18823
-
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranose 1-phosphate
-
r
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate Lachnoclostridium phytofermentans
-
3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranose 1-phosphate
-
r
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate Lachnoclostridium phytofermentans DSM 18823
-
3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranose 1-phosphate
-
r

Organism

Organism UniProt Comment Textmining
Lachnoclostridium phytofermentans A9KSL6
-
-
Lachnoclostridium phytofermentans DSM 18823 A9KSL6
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Lachnoclostridium phytofermentans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
-
Lachnoclostridium phytofermentans 3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranose 1-phosphate
-
r
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
-
Lachnoclostridium phytofermentans DSM 18823 3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranose 1-phosphate
-
r
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranosyl 1-phosphate
-
Lachnoclostridium phytofermentans 3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
-
r
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranosyl 1-phosphate
-
Lachnoclostridium phytofermentans DSM 18823 3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
-
r
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
-
Lachnoclostridium phytofermentans 3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranose 1-phosphate
-
r
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
-
Lachnoclostridium phytofermentans DSM 18823 3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranose 1-phosphate
-
r
3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranose 1-phosphate 3-O-alpha-D-glucopyranosyl-D-glucopyranose i.e. nigerose Lachnoclostridium phytofermentans 3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
-
ir
3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranose 1-phosphate 3-O-alpha-D-glucopyranosyl-D-glucopyranose i.e. nigerose Lachnoclostridium phytofermentans DSM 18823 3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
-
ir
kojibiose + beta-D-glucopyranose 1-phosphate
-
Lachnoclostridium phytofermentans ? + phosphate
-
?
maltose + beta-D-glucopyranose 1-phosphate
-
Lachnoclostridium phytofermentans ? + phosphate
-
?
additional information the enzyme does not significantly phosphorylyze trehalose, kojibiose, nigerose, and maltose Lachnoclostridium phytofermentans ?
-
?
additional information the enzyme does not significantly phosphorylyze trehalose, kojibiose, nigerose, and maltose Lachnoclostridium phytofermentans DSM 18823 ?
-
?

Synonyms

Synonyms Comment Organism
Cphy_3314 locus name Lachnoclostridium phytofermentans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7
-
maltose pH 7.0, 30°C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate Lachnoclostridium phytofermentans
3.5
-
kojibiose pH 7.0, 30°C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate Lachnoclostridium phytofermentans
5.1
-
beta-D-glucopyranose 1-phosphate pH 7.0, 30°C, reverse phosphorolysis reaction, acceptor 3-O-alpha-D-glucopyranosyl-D-glucopyranose, i.e. nigerose Lachnoclostridium phytofermentans
8.5
-
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose pH 7.0, 30°C, phosphorolysis reaction, release of beta-D-glucopyranose 1-phosphate Lachnoclostridium phytofermentans
8.7
-
3-O-alpha-D-glucopyranosyl-D-glucopyranose pH 7.0, 30°C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate Lachnoclostridium phytofermentans
11
-
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose pH 7.0, 30°C, phosphorolysis reaction, release of beta-D-glucopyranose 1-phosphate Lachnoclostridium phytofermentans
17
-
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose pH 7.0, 30°C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate Lachnoclostridium phytofermentans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
synthetic acitvity Lachnoclostridium phytofermentans
8
-
phosphorolytic activity Lachnoclostridium phytofermentans

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 9.5
-
Lachnoclostridium phytofermentans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.069
-
kojibiose pH 7.0, 30°C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate Lachnoclostridium phytofermentans
0.5
-
maltose pH 7.0, 30°C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate Lachnoclostridium phytofermentans
1.4
-
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose pH 7.0, 30°C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate Lachnoclostridium phytofermentans
1.6
-
beta-D-glucopyranose 1-phosphate pH 7.0, 30°C, reverse phosphorolysis reaction, acceptor 3-O-alpha-D-glucopyranosyl-D-glucopyranose, i.e. nigerose Lachnoclostridium phytofermentans
2.8
-
3-O-alpha-D-glucopyranosyl-D-glucopyranose pH 7.0, 30°C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate Lachnoclostridium phytofermentans