Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 | Lachnoclostridium phytofermentans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | 2 | 3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose | pH 7.0, 30°C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate | Lachnoclostridium phytofermentans | |
2.6 | - |
phosphate | pH 7.0, 30°C, phosphorolysis reaction of 3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose, release of beta-D-glucopyranose 1-phosphate | Lachnoclostridium phytofermentans | |
2.9 | - |
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose | pH 7.0, 30°C, phosphorolysis reaction, release of beta-D-glucopyranose 1-phosphate | Lachnoclostridium phytofermentans | |
3.1 | - |
3-O-alpha-D-glucopyranosyl-D-glucopyranose | pH 7.0, 30°C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate | Lachnoclostridium phytofermentans | |
3.2 | - |
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose | pH 7.0, 30°C, phosphorolysis reaction, release of beta-D-glucopyranose 1-phosphate | Lachnoclostridium phytofermentans | |
3.8 | - |
maltose | pH 7.0, 30°C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate | Lachnoclostridium phytofermentans | |
8.3 | - |
beta-D-glucopyranose 1-phosphate | pH 7.0, 30°C, reverse phosphorolysis reaction, acceptor 3-O-alpha-D-glucopyranosyl-D-glucopyranose, i.e. nigerose | Lachnoclostridium phytofermentans | |
11 | - |
phosphate | pH 7.0, 30°C, phosphorolysis reaction of 3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose, release of beta-D-glucopyranose 1-phosphate | Lachnoclostridium phytofermentans | |
50 | - |
kojibiose | pH 7.0, 30°C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate | Lachnoclostridium phytofermentans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Lachnoclostridium phytofermentans | 5829 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate | Lachnoclostridium phytofermentans | - |
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranose 1-phosphate | - |
r | |
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate | Lachnoclostridium phytofermentans DSM 18823 | - |
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranose 1-phosphate | - |
r | |
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate | Lachnoclostridium phytofermentans | - |
3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranose 1-phosphate | - |
r | |
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate | Lachnoclostridium phytofermentans DSM 18823 | - |
3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranose 1-phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lachnoclostridium phytofermentans | A9KSL6 | - |
- |
Lachnoclostridium phytofermentans DSM 18823 | A9KSL6 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Lachnoclostridium phytofermentans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate | - |
Lachnoclostridium phytofermentans | 3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranose 1-phosphate | - |
r | |
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate | - |
Lachnoclostridium phytofermentans DSM 18823 | 3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranose 1-phosphate | - |
r | |
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranosyl 1-phosphate | - |
Lachnoclostridium phytofermentans | 3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate | - |
r | |
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranosyl 1-phosphate | - |
Lachnoclostridium phytofermentans DSM 18823 | 3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate | - |
r | |
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate | - |
Lachnoclostridium phytofermentans | 3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranose 1-phosphate | - |
r | |
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate | - |
Lachnoclostridium phytofermentans DSM 18823 | 3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranose 1-phosphate | - |
r | |
3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranose 1-phosphate | 3-O-alpha-D-glucopyranosyl-D-glucopyranose i.e. nigerose | Lachnoclostridium phytofermentans | 3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate | - |
ir | |
3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranose 1-phosphate | 3-O-alpha-D-glucopyranosyl-D-glucopyranose i.e. nigerose | Lachnoclostridium phytofermentans DSM 18823 | 3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate | - |
ir | |
kojibiose + beta-D-glucopyranose 1-phosphate | - |
Lachnoclostridium phytofermentans | ? + phosphate | - |
? | |
maltose + beta-D-glucopyranose 1-phosphate | - |
Lachnoclostridium phytofermentans | ? + phosphate | - |
? | |
additional information | the enzyme does not significantly phosphorylyze trehalose, kojibiose, nigerose, and maltose | Lachnoclostridium phytofermentans | ? | - |
? | |
additional information | the enzyme does not significantly phosphorylyze trehalose, kojibiose, nigerose, and maltose | Lachnoclostridium phytofermentans DSM 18823 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Cphy_3314 | locus name | Lachnoclostridium phytofermentans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.7 | - |
maltose | pH 7.0, 30°C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate | Lachnoclostridium phytofermentans | |
3.5 | - |
kojibiose | pH 7.0, 30°C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate | Lachnoclostridium phytofermentans | |
5.1 | - |
beta-D-glucopyranose 1-phosphate | pH 7.0, 30°C, reverse phosphorolysis reaction, acceptor 3-O-alpha-D-glucopyranosyl-D-glucopyranose, i.e. nigerose | Lachnoclostridium phytofermentans | |
8.5 | - |
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose | pH 7.0, 30°C, phosphorolysis reaction, release of beta-D-glucopyranose 1-phosphate | Lachnoclostridium phytofermentans | |
8.7 | - |
3-O-alpha-D-glucopyranosyl-D-glucopyranose | pH 7.0, 30°C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate | Lachnoclostridium phytofermentans | |
11 | - |
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose | pH 7.0, 30°C, phosphorolysis reaction, release of beta-D-glucopyranose 1-phosphate | Lachnoclostridium phytofermentans | |
17 | - |
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose | pH 7.0, 30°C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate | Lachnoclostridium phytofermentans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
synthetic acitvity | Lachnoclostridium phytofermentans |
8 | - |
phosphorolytic activity | Lachnoclostridium phytofermentans |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9.5 | - |
Lachnoclostridium phytofermentans |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.069 | - |
kojibiose | pH 7.0, 30°C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate | Lachnoclostridium phytofermentans | |
0.5 | - |
maltose | pH 7.0, 30°C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate | Lachnoclostridium phytofermentans | |
1.4 | - |
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose | pH 7.0, 30°C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate | Lachnoclostridium phytofermentans | |
1.6 | - |
beta-D-glucopyranose 1-phosphate | pH 7.0, 30°C, reverse phosphorolysis reaction, acceptor 3-O-alpha-D-glucopyranosyl-D-glucopyranose, i.e. nigerose | Lachnoclostridium phytofermentans | |
2.8 | - |
3-O-alpha-D-glucopyranosyl-D-glucopyranose | pH 7.0, 30°C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate | Lachnoclostridium phytofermentans |