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2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
beta-D-glucose 1-phosphate + sn-glycerol
beta-D-glucose 1-phosphate + sn-glycerol
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
additional information
?
-
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate

beta-D-glucose 1-phosphate + sn-glycerol
-
-
-
r
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
beta-D-glucose 1-phosphate + sn-glycerol
-
-
-
-
r
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
beta-D-glucose 1-phosphate + sn-glycerol
mechanism: an oxygen atom of phosphate attacks the anomeric C1 atom, and Glu475 works as a general acid
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-
r
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
beta-D-glucose 1-phosphate + sn-glycerol
-
-
-
r
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
beta-D-glucose 1-phosphate + sn-glycerol
mechanism: an oxygen atom of phosphate attacks the anomeric C1 atom, and Glu475 works as a general acid
-
-
r
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
beta-D-glucose 1-phosphate + sn-glycerol
-
-
-
-
r
beta-D-glucose 1-phosphate + sn-glycerol

2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
-
-
-
-
beta-D-glucose 1-phosphate + sn-glycerol
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
-
-
-
-
-
beta-D-glucose 1-phosphate + sn-glycerol
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
-
-
-
r
beta-D-glucose 1-phosphate + sn-glycerol
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
-
-
-
-
r
beta-D-glucose 1-phosphate + sn-glycerol
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
-
-
-
r
beta-D-glucose 1-phosphate + sn-glycerol
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
-
-
-
-
r
additional information

?
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-
1-butanol, 2-butanol, cyclohexanol, benzyl alcohol, hydroquinone, phenol, ethylene glycol, glycerol, erythritol, xylitol D-arabinitol, L-arabinitol, and D-glucitol are no substitutes for sn-glycerol. With beta-D-glucose 1-phosphate as donor the following compounds are no acceptors: D-glucose, D-mannose, D-allose, D-galactose, D-altrose, D-talose, and D-fructose, D-xylose, D-lyxose, D-ribose, D-arabinose, and L-arabinose, D-glucosamine, D-galactosamine, N-acetyl-D-glucosamine, N-acetyl-D-mannosamine, N-acetyl-D-galactosamine, D-glucuronic acid, D-galacturonic acid, 1,5-anhydro-D-glucitol, 2-deoxy-D-glucose, L-rhamnose, alpha-D-glucose 1-phosphate, D-glucose 6-phosphate, 3-Omethyl-D-glucose, methyl-alpha-D-glucoside, and methyl-beta-D-glucoside, trehalose, kojibiose, nigerose, maltose, isomaltose, sucrose, sophorose, laminaribiose, cellobiose, gentiobiose, lactose, lactulose, melibiose, xylobiose, beta-1,4-mannobiose, N,N'-diacetylchitobiose
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additional information
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in the absence of sn-glycerol the enzyme catalyzes the hydrolysis of beta-D-glucose 1-phosphate to alpha-D-glucose and phosphate. This reaction can be explained as a glycosyl transfer reaction to a water molecule
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additional information
?
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in the absence of sn-glycerol the enzyme catalyzes the hydrolysis of beta-D-glucose 1-phosphate to alpha-D-glucose and phosphate. This reaction can be explained as a glycosyl transfer reaction to a water molecule
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additional information
?
-
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1-butanol, 2-butanol, cyclohexanol, benzyl alcohol, hydroquinone, phenol, ethylene glycol, glycerol, erythritol, xylitol D-arabinitol, L-arabinitol, and D-glucitol are no substitutes for sn-glycerol. With beta-D-glucose 1-phosphate as donor the following compounds are no acceptors: D-glucose, D-mannose, D-allose, D-galactose, D-altrose, D-talose, and D-fructose, D-xylose, D-lyxose, D-ribose, D-arabinose, and L-arabinose, D-glucosamine, D-galactosamine, N-acetyl-D-glucosamine, N-acetyl-D-mannosamine, N-acetyl-D-galactosamine, D-glucuronic acid, D-galacturonic acid, 1,5-anhydro-D-glucitol, 2-deoxy-D-glucose, L-rhamnose, alpha-D-glucose 1-phosphate, D-glucose 6-phosphate, 3-Omethyl-D-glucose, methyl-alpha-D-glucoside, and methyl-beta-D-glucoside, trehalose, kojibiose, nigerose, maltose, isomaltose, sucrose, sophorose, laminaribiose, cellobiose, gentiobiose, lactose, lactulose, melibiose, xylobiose, beta-1,4-mannobiose, N,N'-diacetylchitobiose
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Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
beta-D-glucose 1-phosphate + sn-glycerol
beta-D-glucose 1-phosphate + sn-glycerol
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate

beta-D-glucose 1-phosphate + sn-glycerol
D6XZ22
-
-
-
r
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
beta-D-glucose 1-phosphate + sn-glycerol
-
-
-
-
r
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
beta-D-glucose 1-phosphate + sn-glycerol
D6XZ22
-
-
-
r
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
beta-D-glucose 1-phosphate + sn-glycerol
-
-
-
-
r
beta-D-glucose 1-phosphate + sn-glycerol

2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
D6XZ22
-
-
-
r
beta-D-glucose 1-phosphate + sn-glycerol
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
-
-
-
-
r
beta-D-glucose 1-phosphate + sn-glycerol
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
D6XZ22
-
-
-
r
beta-D-glucose 1-phosphate + sn-glycerol
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
-
-
-
-
r
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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Touhara, K.K.; Nihira, T.; Kitaoka, M.; Nakai1, H.; Fushinobu, S.
Structural basis for reversible phosphorolysis and hydrolysis reactions of 2-O-alpha-glucosylglycerol phosphorylase
J. Biol. Chem.
289
18067-18075
2014
Bacillus selenitireducens (D6XZ22), Bacillus selenitireducens DSM 15326 (D6XZ22)
brenda
Nihira, T.; Saito, Y.; Ohtsubo, K.-i.; , Nakai1, H.; Kitaoka, M.
2-O-alpha-D-Glucosylglycerol phosphorylase from Bacillus selenitireducens MLS10 possessing hydrolytic activity on beta-D-glucose 1-phosphate
PLoS One
9
e86548
2014
Bacillus selenitireducens (D6XZ22), Bacillus selenitireducens DSM 15326 (D6XZ22)
brenda