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Enzyme Nomenclature
Information on EC 2.4.1.332 - 1,2-alpha-glucosylglycerol phosphorylase
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The expected taxonomic range for this enzyme is: Bacillus selenitireducens
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EC NUMBER 
COMMENTARY 
2.4.1.332
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RECOMMENDED NAME
GeneOntology No.
1,2-alpha-glucosylglycerol phosphorylase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
2-O-alpha-D-glucopyranosyl-glycerol + phosphate = beta-D-glucose 1-phosphate + glycerol
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
phospho group transfer
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SYSTEMATIC NAME
IUBMB Comments
2-O-alpha-D-glucopyranosyl-sn-glycerol:phosphate beta-D-glucosyltransferase
The enzyme has been isolated from the bacterium Bacillus selenitireducens. In the absence of glycerol the enzyme produces alpha-D-glucopyranose and phosphate from beta-D-glucopyranose 1-phosphate. In this reaction the glucosyl residue is transferred to a water molecule with an inversion of the anomeric conformation.
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
beta-D-glucose 1-phosphate + sn-glycerol
beta-D-glucose 1-phosphate + sn-glycerol
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
beta-D-glucose 1-phosphate + sn-glycerol
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r
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
beta-D-glucose 1-phosphate + sn-glycerol
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r
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
beta-D-glucose 1-phosphate + sn-glycerol
mechanism: an oxygen atom of phosphate attacks the anomeric C1 atom, and Glu475 works as a general acid
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r
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
beta-D-glucose 1-phosphate + sn-glycerol
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-
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r
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
beta-D-glucose 1-phosphate + sn-glycerol
mechanism: an oxygen atom of phosphate attacks the anomeric C1 atom, and Glu475 works as a general acid
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r
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
beta-D-glucose 1-phosphate + sn-glycerol
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r
beta-D-glucose 1-phosphate + sn-glycerol
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
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beta-D-glucose 1-phosphate + sn-glycerol
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
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beta-D-glucose 1-phosphate + sn-glycerol
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
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r
beta-D-glucose 1-phosphate + sn-glycerol
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
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r
beta-D-glucose 1-phosphate + sn-glycerol
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
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r
beta-D-glucose 1-phosphate + sn-glycerol
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
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r
additional information
?
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1-butanol, 2-butanol, cyclohexanol, benzyl alcohol, hydroquinone, phenol, ethylene glycol, glycerol, erythritol, xylitol D-arabinitol, L-arabinitol, and D-glucitol are no substitutes for sn-glycerol. With beta-D-glucose 1-phosphate as donor the following compounds are no acceptors: D-glucose, D-mannose, D-allose, D-galactose, D-altrose, D-talose, and D-fructose, D-xylose, D-lyxose, D-ribose, D-arabinose, and L-arabinose, D-glucosamine, D-galactosamine, N-acetyl-D-glucosamine, N-acetyl-D-mannosamine, N-acetyl-D-galactosamine, D-glucuronic acid, D-galacturonic acid, 1,5-anhydro-D-glucitol, 2-deoxy-D-glucose, L-rhamnose, alpha-D-glucose 1-phosphate, D-glucose 6-phosphate, 3-Omethyl-D-glucose, methyl-alpha-D-glucoside, and methyl-beta-D-glucoside, trehalose, kojibiose, nigerose, maltose, isomaltose, sucrose, sophorose, laminaribiose, cellobiose, gentiobiose, lactose, lactulose, melibiose, xylobiose, beta-1,4-mannobiose, N,N'-diacetylchitobiose
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additional information
?
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in the absence of sn-glycerol the enzyme catalyzes the hydrolysis of beta-D-glucose 1-phosphate to alpha-D-glucose and phosphate. This reaction can be explained as a glycosyl transfer reaction to a water molecule
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additional information
?
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in the absence of sn-glycerol the enzyme catalyzes the hydrolysis of beta-D-glucose 1-phosphate to alpha-D-glucose and phosphate. This reaction can be explained as a glycosyl transfer reaction to a water molecule
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additional information
?
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1-butanol, 2-butanol, cyclohexanol, benzyl alcohol, hydroquinone, phenol, ethylene glycol, glycerol, erythritol, xylitol D-arabinitol, L-arabinitol, and D-glucitol are no substitutes for sn-glycerol. With beta-D-glucose 1-phosphate as donor the following compounds are no acceptors: D-glucose, D-mannose, D-allose, D-galactose, D-altrose, D-talose, and D-fructose, D-xylose, D-lyxose, D-ribose, D-arabinose, and L-arabinose, D-glucosamine, D-galactosamine, N-acetyl-D-glucosamine, N-acetyl-D-mannosamine, N-acetyl-D-galactosamine, D-glucuronic acid, D-galacturonic acid, 1,5-anhydro-D-glucitol, 2-deoxy-D-glucose, L-rhamnose, alpha-D-glucose 1-phosphate, D-glucose 6-phosphate, 3-Omethyl-D-glucose, methyl-alpha-D-glucoside, and methyl-beta-D-glucoside, trehalose, kojibiose, nigerose, maltose, isomaltose, sucrose, sophorose, laminaribiose, cellobiose, gentiobiose, lactose, lactulose, melibiose, xylobiose, beta-1,4-mannobiose, N,N'-diacetylchitobiose
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
beta-D-glucose 1-phosphate + sn-glycerol
beta-D-glucose 1-phosphate + sn-glycerol
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
beta-D-glucose 1-phosphate + sn-glycerol
D6XZ22
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r
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
beta-D-glucose 1-phosphate + sn-glycerol
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r
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
beta-D-glucose 1-phosphate + sn-glycerol
D6XZ22
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r
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
beta-D-glucose 1-phosphate + sn-glycerol
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r
beta-D-glucose 1-phosphate + sn-glycerol
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
D6XZ22
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r
beta-D-glucose 1-phosphate + sn-glycerol
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
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r
beta-D-glucose 1-phosphate + sn-glycerol
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
D6XZ22
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r
beta-D-glucose 1-phosphate + sn-glycerol
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
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r
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
beta-D-glucose 1-phosphate
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the Km for beta-D-glucose 1-phosphate in the hydrolytic reaction is 0.041 and 0.16 mM at pH 6.0 and pH 7.5 respectively
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
beta-D-glucose 1-phosphate
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kcat for beta-D-glucose 1-phosphate in the hydrolytic reaction is 2.8 and 1.7 s(-1) at pH 6.0 and pH 7.5 respectively
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
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hydrolysis of beta-D-glucose 1-phosphate has an optimum of pH 6.0
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PDB
SCOP
CATH
ORGANISM
UNIPROT
Bacillus selenitireducens (strain ATCC 700615 / DSM 15326 / MLS10)
Bacillus selenitireducens (strain ATCC 700615 / DSM 15326 / MLS10)
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
dimer
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Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
obtained at 20ºC using the hanging drop vapor diffusion method by mixing 1 microL of protein solution containing 4.3–5.7 mg/mL protein with an equal volume of reservoir solution containing 28% polyethylene glycol 400, 0.1 M HEPES-NaOH (pH 7.5), and 0.2 M CaCl2
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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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LINKS TO OTHER DATABASES (specific for EC-Number 2.4.1.332)
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