Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Salisediminibacterium selenitireducens |
Crystallization (Comment) | Organism |
---|---|
obtained at 20ΒΊC using the hanging drop vapor diffusion method by mixing 1 microL of protein solution containing 4.3Β5.7 mg/mL protein with an equal volume of reservoir solution containing 28% polyethylene glycol 400, 0.1 M HEPES-NaOH (pH 7.5), and 0.2 M CaCl2 | Salisediminibacterium selenitireducens |
Protein Variants | Comment | Organism |
---|---|---|
E475Q | inactive mutant | Salisediminibacterium selenitireducens |
K587A | inactive mutant | Salisediminibacterium selenitireducens |
W381F | inactive mutant | Salisediminibacterium selenitireducens |
Y327F | inactive mutant | Salisediminibacterium selenitireducens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate | Salisediminibacterium selenitireducens | - |
beta-D-glucose 1-phosphate + sn-glycerol | - |
r | |
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate | Salisediminibacterium selenitireducens DSM 15326 | - |
beta-D-glucose 1-phosphate + sn-glycerol | - |
r | |
beta-D-glucose 1-phosphate + sn-glycerol | Salisediminibacterium selenitireducens | - |
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate | - |
r | |
beta-D-glucose 1-phosphate + sn-glycerol | Salisediminibacterium selenitireducens DSM 15326 | - |
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salisediminibacterium selenitireducens | D6XZ22 | - |
- |
Salisediminibacterium selenitireducens DSM 15326 | D6XZ22 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate | - |
Salisediminibacterium selenitireducens | beta-D-glucose 1-phosphate + sn-glycerol | - |
r | |
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate | mechanism: an oxygen atom of phosphate attacks the anomeric C1 atom, and Glu475 works as a general acid | Salisediminibacterium selenitireducens | beta-D-glucose 1-phosphate + sn-glycerol | - |
r | |
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate | - |
Salisediminibacterium selenitireducens DSM 15326 | beta-D-glucose 1-phosphate + sn-glycerol | - |
r | |
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate | mechanism: an oxygen atom of phosphate attacks the anomeric C1 atom, and Glu475 works as a general acid | Salisediminibacterium selenitireducens DSM 15326 | beta-D-glucose 1-phosphate + sn-glycerol | - |
r | |
beta-D-glucose 1-phosphate + sn-glycerol | - |
Salisediminibacterium selenitireducens | 2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate | - |
? | |
beta-D-glucose 1-phosphate + sn-glycerol | - |
Salisediminibacterium selenitireducens | 2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate | - |
r | |
beta-D-glucose 1-phosphate + sn-glycerol | - |
Salisediminibacterium selenitireducens DSM 15326 | 2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate | - |
? | |
beta-D-glucose 1-phosphate + sn-glycerol | - |
Salisediminibacterium selenitireducens DSM 15326 | 2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate | - |
r | |
additional information | in the absence of sn-glycerol the enzyme catalyzes the hydrolysis of beta-D-glucose 1-phosphate to alpha-D-glucose and phosphate. This reaction can be explained as a glycosyl transfer reaction to a water molecule | Salisediminibacterium selenitireducens | ? | - |
? | |
additional information | in the absence of sn-glycerol the enzyme catalyzes the hydrolysis of beta-D-glucose 1-phosphate to alpha-D-glucose and phosphate. This reaction can be explained as a glycosyl transfer reaction to a water molecule | Salisediminibacterium selenitireducens DSM 15326 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
2-O-alpha-D-glucopyranosylglycerol phosphorylase | - |
Salisediminibacterium selenitireducens |
Bsel_2816 | locus name | Salisediminibacterium selenitireducens |