Information on EC 2.4.1.219 - vomilenine glucosyltransferase

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The expected taxonomic range for this enzyme is: Rauvolfia

EC NUMBER
COMMENTARY hide
2.4.1.219
-
RECOMMENDED NAME
GeneOntology No.
vomilenine glucosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-glucose + vomilenine = UDP + raucaffricine
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycosyl group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
ajmaline and sarpagine biosynthesis
-
-
Biosynthesis of secondary metabolites
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Indole alkaloid biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
UDP-glucose:vomilenine 21-O-beta-D-glucosyltransferase
The indole alkaloid raucaffricine accumulates during the culture of Rauvolfia cell suspensions.
CAS REGISTRY NUMBER
COMMENTARY hide
102925-37-1
-
9031-48-5
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
Rauwolfia caffra
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-
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
strictosidine + UDP
?
show the reaction diagram
-
recombinant enzyme
-
-
?
UDP-glucose + vomilenine
UDP + raucaffricine
show the reaction diagram
UDP-glucose + vomilenine
UDP-glucose + raucaffricine
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-glucose + vomilenine
UDP + raucaffricine
show the reaction diagram
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(phenylmethyl)sulfonyl fluoride
-
strong inhibition at 10 mM
1,10-phenanthroline
-
strong inhibition at 5 mM
5,5'-dithiobis(2-nitrobenzoic acid)
-
strong inhibition at 1.25 mM
Cu2+
-
complete inhibition at 10 mM
D-fructose
-
-
EDTA
-
strong inhibition at 5 mM
Fe3+
-
complete inhibition at 10 mM
Hg2+
-
complete inhibition at 10 mM
p-Hydroxybenzaldehyde
-
strong inhibition at 5 mM
Zn2+
-
complete inhibition at 10 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.3
Raucaffricine
-
-
1.8
strictosidine
-
-
0.8
UDP-glucose
-
-
0.04
Vomilenine
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
61000
-
gel filtration
66600
-
gel filtration
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
no glycosylation
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
55
-
30 min, 50% of activity left
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
2-mercaptoethanol stabilizes transferase activity
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ascorbic acid stabilizes transferase activity
-
dithioerythritol stabilizes transferase activity
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 3 weeks, no loss in activity
-
4°C, 6 month, 30% loss in activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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