Information on EC 2.4.1.192 - nuatigenin 3beta-glucosyltransferase

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The expected taxonomic range for this enzyme is: Avena sativa

EC NUMBER
COMMENTARY hide
2.4.1.192
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RECOMMENDED NAME
GeneOntology No.
nuatigenin 3beta-glucosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-glucose + (20S,22S,25S)-22,25-epoxyfurost-5-ene-3beta,26-diol = UDP + (20S,22S,25S)-22,25-epoxyfurost-5-ene-3beta,26-diol 3-O-beta-D-glucoside
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
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SYSTEMATIC NAME
IUBMB Comments
UDP-glucose:(20S,22S,25S)-22,25-epoxyfurost-5-ene-3beta,26-diol 3-O-beta-D-glucosyltransferase
Some other sapogenins can act as glucosyl acceptors. Involved in the biosynthesis of plant saponins. Not identical with EC 2.4.1.173 (sterol 3beta-glucosyltransferase) or EC 2.4.1.193 (sarsapogenin 3beta-glucosyltransferase).
CAS REGISTRY NUMBER
COMMENTARY hide
108891-57-2
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(25R)-5alpha-spirostan-3beta-ol + UDP-glucose
UDP + (25R)-5alpha-spirostan-3beta-ol 3-O-beta-D-glucoside
show the reaction diagram
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i.e. tigogenin, at 12% of the activity with nuatigenin
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-
?
(25R)-5alpha-spirostane-3beta,6alpha-diol + UDP-glucose
UDP + (25R)-5alpha-spirostane-3beta,6alpha-diol 3-O-beta-D-glucoside
show the reaction diagram
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i.e. chlorogenin, at 30% of the activity with nuatigenin
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-
?
(25R)-spirost-5-en-3beta-ol + UDP-glucose
UDP + (25R)-spirost-5-en-3beta-ol 3-O-beta-D-glucoside
show the reaction diagram
20S,22S,25S-spirost-5-ene-3beta,25-diol + UDP-glucose
UDP + 20S,22S,25S-spirost-5-ene-3beta,25-diol 3-O-beta-D-glucoside
show the reaction diagram
22S,25S-5alpha-tomatanin-3beta-ol + UDP-glucose
UDP + 22S,25S-5alpha-tomatanin-3beta-ol 3-O-beta-D-glucoside
show the reaction diagram
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i.e. tomatidine, at 57% of the activity with nuatigenin
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-
?
22S,25S-solanid-5-enin-3beta-ol + UDP-glucose
UDP + gamma-chaconine
show the reaction diagram
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i.e. solanidine, at 39% of the activity with nuatigenin
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?
androst-5-en-3beta-ol-17-one + UDP-glucose
UDP + androst-5-en-3beta-ol-17-one 3-O-beta-D-glucoside
show the reaction diagram
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i.e. androsterone, at 15% of the activity with nuatigenin
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pregn-5-en-3beta-ol-20-one + UDP-glucose
UDP + pregn-5-en-3beta-ol-20-one 3-O-beta-D-glucoside
show the reaction diagram
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i.e. pregnenolone, at 26% of the activity with nuatigenin
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?
UDP-glucose + (20S,22S,25S)-22,25-epoxyfurost-5-ene-3beta,26-diol
UDP + (20S,22S,25S)-22,25-epoxyfurost-5-ene-3beta,26-diol 3-O-beta-D-glucoside
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-glucose + (20S,22S,25S)-22,25-epoxyfurost-5-ene-3beta,26-diol
UDP + (20S,22S,25S)-22,25-epoxyfurost-5-ene-3beta,26-diol 3-O-beta-D-glucoside
show the reaction diagram
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participates in the initiation of sugar chain formation during the biosynthesis of oat saponins: avenacosides A and B
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?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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no stimulation by divalent cations: Ca2+, Mn2+ or Mg2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
p-chloromercuribenzoate
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Triton X-100
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 8.5
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7.5 - 8.5
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.8 - 8.5
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at pH 5.8 about 50% of activity maximum, at pH 6.5-8.5 activity maximum
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30 - 39
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at 30°C activity maximum, at 39°C activity is 7times lower than at 30°C
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
58000 - 62000
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gel filtration
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45
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unstable
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
2-mercaptoethanol, 10 mM, improves stability in solution, 35% loss of activity after 24 h at 4°C
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rather unstable when dissolved in buffer, 65% loss of activity after 24 h at 4°C
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, cytosolic fraction stable for several months
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-20°C, in the form of dry acetone powder, stable for several weeks
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial
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