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Literature summary for 2.4.1.192 extracted from

  • Kalinowska, M.; Wojciechowski, Z.A.
    Substrate specificity of partially purified UDP-glucose:nuatigenin glucosyltransferase from oat leaves (1988), Plant Sci., 55, 239-245.
No PubMed abstract available
Search for more literature for 2.4.1.192

General Stability

General Stability Organism
2-mercaptoethanol, 10 mM, improves stability in solution, 35% loss of activity after 24 h at 4°C Avena sativa
rather unstable when dissolved in buffer, 65% loss of activity after 24 h at 4°C Avena sativa

Inhibitors

Inhibitors Comment Organism Structure
Hg2+
-
 Avena sativa
p-chloromercuribenzoate
-
 Avena sativa
Triton X-100
-
 Avena sativa
UDP
-
 Avena sativa
UMP
-
 Avena sativa
Zn2+
-
 Avena sativa

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Avena sativa 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000 62000 gel filtration Avena sativa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-glucose + (20S,22S,25S)-22,25-epoxyfurost-5-ene-3beta,26-diol Avena sativa participates in the initiation of sugar chain formation during the biosynthesis of oat saponins: avenacosides A and B UDP + (20S,22S,25S)-22,25-epoxyfurost-5-ene-3beta,26-diol 3-O-beta-D-glucoside
-
 ?

Organism

Organism UniProt Comment Textmining
Avena sativa
-
 oat
-

Purification (Commentary)

Purification (Comment) Organism
partial Avena sativa

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Avena sativa
-

Storage Stability

Storage Stability Organism
-20°C, in the form of dry acetone powder, stable for several weeks Avena sativa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(25R)-5alpha-spirostan-3beta-ol + UDP-glucose i.e. tigogenin, at 12% of the activity with nuatigenin Avena sativa UDP + (25R)-5alpha-spirostan-3beta-ol 3-O-beta-D-glucoside
-
 ?
(25R)-5alpha-spirostane-3beta,6alpha-diol + UDP-glucose i.e. chlorogenin, at 30% of the activity with nuatigenin Avena sativa UDP + (25R)-5alpha-spirostane-3beta,6alpha-diol 3-O-beta-D-glucoside
-
 ?
(25R)-spirost-5-en-3beta-ol + UDP-glucose i.e. diosgenin, at 17% of the activity with nuatigenin Avena sativa UDP + (25R)-spirost-5-en-3beta-ol 3-O-beta-D-glucoside
-
 ?
20S,22S,25S-spirost-5-ene-3beta,25-diol + UDP-glucose i.e. isonuatigenin, at 57% of the activity with nuatigenin Avena sativa UDP + 20S,22S,25S-spirost-5-ene-3beta,25-diol 3-O-beta-D-glucoside
-
 ?
22S,25S-5alpha-tomatanin-3beta-ol + UDP-glucose i.e. tomatidine, at 57% of the activity with nuatigenin Avena sativa UDP + 22S,25S-5alpha-tomatanin-3beta-ol 3-O-beta-D-glucoside
-
 ?
22S,25S-solanid-5-enin-3beta-ol + UDP-glucose i.e. solanidine, at 39% of the activity with nuatigenin Avena sativa UDP + gamma-chaconine
-
 ?
androst-5-en-3beta-ol-17-one + UDP-glucose i.e. androsterone, at 15% of the activity with nuatigenin Avena sativa UDP + androst-5-en-3beta-ol-17-one 3-O-beta-D-glucoside
-
 ?
pregn-5-en-3beta-ol-20-one + UDP-glucose i.e. pregnenolone, at 26% of the activity with nuatigenin Avena sativa UDP + pregn-5-en-3beta-ol-20-one 3-O-beta-D-glucoside
-
 ?
UDP-glucose + (20S,22S,25S)-22,25-epoxyfurost-5-ene-3beta,26-diol i.e. nuatigenin, TDPglucose at 8%, ADPglucose at 11%, UDPgalactose at 40% the rate of UDP-glucose Avena sativa UDP + (20S,22S,25S)-22,25-epoxyfurost-5-ene-3beta,26-diol 3-O-beta-D-glucoside i.e. nuatigenin 3beta-D-monoglucoside ?
UDP-glucose + (20S,22S,25S)-22,25-epoxyfurost-5-ene-3beta,26-diol participates in the initiation of sugar chain formation during the biosynthesis of oat saponins: avenacosides A and B Avena sativa UDP + (20S,22S,25S)-22,25-epoxyfurost-5-ene-3beta,26-diol 3-O-beta-D-glucoside
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Avena sativa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 unstable Avena sativa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 8.5
-
 Avena sativa