Information on EC 2.4.1.183 - alpha-1,3-glucan synthase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
2.4.1.183
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RECOMMENDED NAME
GeneOntology No.
alpha-1,3-glucan synthase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-glucose + [alpha-D-glucosyl-(1->3)]n = UDP + [alpha-D-glucosyl-(1->3)]n+1
show the reaction diagram
a glucan primer is needed to start the reaction, which brings about elongation of the glucan chains
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
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SYSTEMATIC NAME
IUBMB Comments
UDP-glucose:alpha-D-(1->3)-glucan 3-alpha-D-glucosyltransferase
A glucan primer is needed to begin the reaction, which brings about elongation of the glucan chains.
CAS REGISTRY NUMBER
COMMENTARY hide
113478-38-9
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
i.e. Emericella nidulans, genes agsA and agsB
UniProt
Manually annotated by BRENDA team
i.e. Emericella nidulans, genes agsA and agsB
UniProt
Manually annotated by BRENDA team
strain N402. Aspergillus niger responds to cell wall stress by transcriptional activation of cell wall reinforcing genes including agsA (encoding a alpha-1,3-glucan synthase) through an RIm1p-like transcription factor.
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Manually annotated by BRENDA team
gene epsF; strain NCC2745
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Manually annotated by BRENDA team
strain NCC2745
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Manually annotated by BRENDA team
AHT strain
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Manually annotated by BRENDA team
AHT strain
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-glucose + 2-(octadecylthio)-ethyl-beta-D-glucose
UDP + 2-(octadecylthio)-ethyl-beta-D-glucose-1,3-alpha-D-glucose
show the reaction diagram
UDP-glucose + [alpha-D-glucosyl-(1-3)]n
UDP + [alpha-D-glucosyl-(1-3)]n+1
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-glucose + [alpha-D-glucosyl-(1-3)]n
UDP + [alpha-D-glucosyl-(1-3)]n+1
show the reaction diagram
additional information
?
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Antiserum against the isolated 1,3-alpha-D-glucan synthase
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glucosyl transfer activity is completely inhibited, not sucrose hydrolysis activity
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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assay development
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant as His-tagged or GST-tagged proteins from Escherichia coli
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cloning of eps gene cluster from genomic library and expression in Escherichia coli as His-tagges or GST-tagged proteins, DNA sequence analysis
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Escherichia coli strain DH5alpha
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expression in Escherichia coli. Isolation of fragments of a family of five 1,3-alpha D-glucan synthase-encoding genes (agsA-E); expression in Escherichia coli. Isolation of fragments of a family of five 1,3-alpha D-glucan synthase-encoding genes (agsA-E); expression in Escherichia coli. Isolation of fragments of a family of five 1,3-alpha D-glucan synthase-encoding genes (agsA-E); expression in Escherichia coli. Isolation of fragments of a family of five 1,3-alpha D-glucan synthase-encoding genes (agsA-E)
expression in Escherichia coli; expression in Escherichia coli; expression in Escherichia coli
genes agsA and agsB, expression analysis
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information