Cloned (Comment) | Organism |
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genes agsA and agsB, expression analysis | Aspergillus nidulans |
Protein Variants | Comment | Organism |
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additional information | construction of several mutant strains in Aspergillus nidulans by gene replacement: agsA disruption, agsB disruption, and double-disruption strains, as well asl CagsB strains in which agsB expression is controlled by the inducible alcA promoter, with or without the agsA-disrupting mutation. The agsA disruption strains do not show markedly different phenotypes from the wild-type strain. The agsB disruption strains forms dispersed hyphal cells under liquid culture conditions, regardless of the agsA genetic background. Dispersed hyphal cells are also observed in liquid culture of the CagsB strains when agsB expression is repressed, whereas these strains grow normally in plate culture even under the agsB-repressed conditions. Phenotypes, overview | Aspergillus nidulans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell wall | - |
Aspergillus nidulans | 5618 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus nidulans | Q5B0P5 | i.e. Emericella nidulans, genes agsA and agsB | - |
Aspergillus nidulans ATCC 38163 | Q5B0P5 | i.e. Emericella nidulans, genes agsA and agsB | - |
Synonyms | Comment | Organism |
---|---|---|
AGS | - |
Aspergillus nidulans |
General Information | Comment | Organism |
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malfunction | alpha-1,3-glucan is the main component of the alkali-soluble cell wall fraction in the wild-type and agsA disruption strains, but almost no alpha-1,3-glucan is found in the alkali-soluble fraction derived from either the agsB disruption strain or the CagsB strain under the agsB-repressed conditions, regardless of the agsA genetic background. Hyphal morphology of the control and agsB disruption strains, overview | Aspergillus nidulans |
additional information | expression profiles of genes involved in cell wall biosynthesis. Levels of transcription of several cell wall-related genes (gelA, chsA, and gfaA) are significantly upregulated in the agsB disruption strain, transcription of gelB and csmB is significantly downregulated in the disruptant. In the CagsB strain, the expression of genes agsA, fksA, gelA, chsA, and gfaA is significantly upregulated under the agsB-repressing conditions, as is the case in the agsB disruption strain for all except fksA. In contrast, under the agsB-inducing conditions, the expression levels of fksA, gelB, chsD, and gfaA are significantly reduced in the CagsB strain, gene csmA is significantly upregulated under these conditions | Aspergillus nidulans |
physiological function | the enzyme is involved in the cell wall biogenesis. Alteration of the alpha-1,3-glucan amount is counterbalanced by alterations in the amount of another cell wall component such as beta-1,3-glucan or chitin | Aspergillus nidulans |