Information on EC 2.4.1.158 - 13-hydroxydocosanoate 13-beta-glucosyltransferase

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The expected taxonomic range for this enzyme is: Pseudohyphozyma bogoriensis

EC NUMBER
COMMENTARY hide
2.4.1.158
-
RECOMMENDED NAME
GeneOntology No.
13-hydroxydocosanoate 13-beta-glucosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-glucose + 13-hydroxydocosanoate = UDP + 13-beta-D-glucosyloxydocosanoate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
UDP-glucose:13-hydroxydocosanoate 13-beta-D-glucosyltransferase
13-beta-D-Glucosyloxydocosanoate can also act as acceptor, leading to the formation by Candida bogoriensis of the extracellular glycolipid, hydroxydocosanoate sophoroside diacetate.
CAS REGISTRY NUMBER
COMMENTARY hide
70457-13-5
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDPglucose + 13-(beta-D-glucopyranosyl)oxydocosanoic acid methyl ester
UDP + 13-[(2'-O-beta-D-glucopyranosyl-beta-D-glucopyranosyl)oxy]docosanoic acid methyl ester
show the reaction diagram
-
glucosyltransferase II
-
?
UDPglucose + 13-hydroxydocosanoic acid
UDP + 13-(beta-D-glucopyranosyl)oxydocosanoic acid
show the reaction diagram
-
glucosyltransferase I
-
?
additional information
?
-
-
enzyme seems to be a single multifunctional protein catalyzing both transferase reactions
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDPglucose + 13-(beta-D-glucopyranosyl)oxydocosanoic acid methyl ester
UDP + 13-[(2'-O-beta-D-glucopyranosyl-beta-D-glucopyranosyl)oxy]docosanoic acid methyl ester
show the reaction diagram
-
glucosyltransferase II
-
?
UDPglucose + 13-hydroxydocosanoic acid
UDP + 13-(beta-D-glucopyranosyl)oxydocosanoic acid
show the reaction diagram
-
glucosyltransferase I
-
-
?
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NaCl
-
0.2 mM: 50% inhibition of glucosyltransferase I, 0.25 mM: 50% inhibition of glucosyltransferase II
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.04
UDPglucose
-
glucosyltransferase I and II
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0222
-
glucosyltransferase I
0.0242
-
glucosyltransferase II
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 8
-
glucosyltransferase I, within this range activity of glucosyltransferase II increases
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 37
-
30C-37C: 100% of activity, at 5C: 10% of maximal activity
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50000
-
gel filtration
52000
-
1 * 52000, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
-
1 * 52000, SDS-PAGE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.9 - 8.1
-
glucosyltransferase I and II: stable
488624
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
glycerol, 20%, stabilizes
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4C, 20% glycerol: half-life 35 days
-
4C, without glycerol: half-life 5 days
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE