General Stability | Organism |
---|---|
glycerol, 20%, stabilizes | Pseudohyphozyma bogoriensis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NaCl | 0.2 mM: 50% inhibition of glucosyltransferase I, 0.25 mM: 50% inhibition of glucosyltransferase II | Pseudohyphozyma bogoriensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
UDPglucose | glucosyltransferase I and II | Pseudohyphozyma bogoriensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
gel filtration | Pseudohyphozyma bogoriensis |
52000 | - |
1 * 52000, SDS-PAGE | Pseudohyphozyma bogoriensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDPglucose + 13-(beta-D-glucopyranosyl)oxydocosanoic acid methyl ester | Pseudohyphozyma bogoriensis | glucosyltransferase II | UDP + 13-[(2'-O-beta-D-glucopyranosyl-beta-D-glucopyranosyl)oxy]docosanoic acid methyl ester | - |
? | |
UDPglucose + 13-hydroxydocosanoic acid | Pseudohyphozyma bogoriensis | glucosyltransferase I | UDP + 13-(beta-D-glucopyranosyl)oxydocosanoic acid | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudohyphozyma bogoriensis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Pseudohyphozyma bogoriensis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0222 | - |
glucosyltransferase I | Pseudohyphozyma bogoriensis |
0.0242 | - |
glucosyltransferase II | Pseudohyphozyma bogoriensis |
Storage Stability | Organism |
---|---|
4°C, 20% glycerol: half-life 35 days | Pseudohyphozyma bogoriensis |
4°C, without glycerol: half-life 5 days | Pseudohyphozyma bogoriensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme seems to be a single multifunctional protein catalyzing both transferase reactions | Pseudohyphozyma bogoriensis | ? | - |
? | |
UDPglucose + 13-(beta-D-glucopyranosyl)oxydocosanoic acid methyl ester | glucosyltransferase II | Pseudohyphozyma bogoriensis | UDP + 13-[(2'-O-beta-D-glucopyranosyl-beta-D-glucopyranosyl)oxy]docosanoic acid methyl ester | - |
? | |
UDPglucose + 13-hydroxydocosanoic acid | glucosyltransferase I | Pseudohyphozyma bogoriensis | UDP + 13-(beta-D-glucopyranosyl)oxydocosanoic acid | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 52000, SDS-PAGE | Pseudohyphozyma bogoriensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Pseudohyphozyma bogoriensis |
30 | 37 | - |
Pseudohyphozyma bogoriensis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
5 | 37 | 30°C-37°C: 100% of activity, at 5°C: 10% of maximal activity | Pseudohyphozyma bogoriensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8 | glucosyltransferase I, within this range activity of glucosyltransferase II increases | Pseudohyphozyma bogoriensis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.9 | 8.1 | glucosyltransferase I and II: stable | Pseudohyphozyma bogoriensis |