Information on EC 2.4.1.153 - UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminyltransferase

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The expected taxonomic range for this enzyme is: Eukaryota, Archaea

EC NUMBER
COMMENTARY hide
2.4.1.153
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RECOMMENDED NAME
GeneOntology No.
UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-N-acetyl-alpha-D-glucosamine + dolichyl phosphate = UDP + dolichyl N-acetyl-alpha-D-glucosaminyl phosphate
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
protein N-glycosylation (Methanococcus voltae)
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SYSTEMATIC NAME
IUBMB Comments
UDP-N-acetyl-D-glucosamine:dolichyl-phosphate alpha-N-acetyl-D-glucosaminyltransferase
The enzyme, characterized from the methanogenic archaeon Methanococcus voltae, initiates N-linked glycosylation in that organism. The enzyme differs from the eukaryotic enzyme, which leaves one additional phosphate group on the dolichyl product (cf. EC 2.7.8.15, UDP-N-acetylglucosamine---dolichyl-phosphate N-acetylglucosaminephosphotransferase).
CAS REGISTRY NUMBER
COMMENTARY hide
63363-73-5
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-alpha-D-glucosamine + dolichyl phosphate
UDP + dolichyl N-acetyl-alpha-D-glucosaminyl phosphate
show the reaction diagram
UDP-N-acetyl-D-glucosamine + dolichyl phosphate
UDP + dolichyl N-acetyl-alpha-D-glucosaminyl phosphate
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-alpha-D-glucosamine + dolichyl phosphate
UDP + dolichyl N-acetyl-alpha-D-glucosaminyl phosphate
show the reaction diagram
UDP-N-acetyl-D-glucosamine + dolichyl phosphate
UDP + dolichyl N-acetyl-alpha-D-glucosaminyl phosphate
show the reaction diagram
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one of the initial steps of the lipid-mediated glycosylation pathway
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?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
enzymatic activity requires divalent metal cations Ca2+, Mn2+, or Mg2+
Divalent cation
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required
Mg2+
enzymatic activity requires divalent metal cations Ca2+, Mn2+, or Mg2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Detergent
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required at low concentrations
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Nonidet P-40
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detergent required
Triton X-100
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detergent required
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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specific activities of dolichyl phosphate-mannosyltransferase are comparable in liver homogenates from normal controls and patients with cystic fibrosis and diabetes mellitus
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
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90% of the activity is particulate
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Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
28175
x * 28175, calculated from sequence
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
overexpression in Escherichia coli with N-terminal T7 and C-terminal His6 tags
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
biotechnology
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use of enzyme gene in combination with tunicamycin as a selection marker for transformation of Arabidopsis, identification of transformants at a very early stage post germinantion