Information on EC 2.4.1.125 - sucrose-1,6-alpha-glucan 3(6)-alpha-glucosyltransferase

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The expected taxonomic range for this enzyme is: Streptococcus

EC NUMBER
COMMENTARY hide
2.4.1.125
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RECOMMENDED NAME
GeneOntology No.
sucrose-1,6-alpha-glucan 3(6)-alpha-glucosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
sucrose + [(1->6)-alpha-D-glucosyl]n = D-fructose + (1->3)-alpha-D-glucosyl[(1->6)-alpha-D-glucosyl]n
show the reaction diagram
(2)
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-
-
sucrose + [(1->6)-alpha-D-glucosyl]n = D-fructose + [(1->6)-alpha-D-glucosyl]n+1
show the reaction diagram
(1)
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-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
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-
-
-
SYSTEMATIC NAME
IUBMB Comments
sucrose:(1->6)-alpha-D-glucan 3(6)-alpha-D-glucosyltransferase
The enzyme was characterized from the dental caries bacterium Streptococcus mutans. It transfers glucosyl residues from sucrose to either the 6- or the 3-positions of glucose residues in glucans, producing a highly-branched extracellular D-glucan polymers that promote attachment of the bacteria to teeth. Three types of the enzyme have been described; the insoluble polymers produced by GTF-I and GTF-SI contain 85% alpha(1->3) bonds and 15% alpha(1->6) bonds, while the soluble polymers produced by GTF-S contain only 30% of alpha(1->3) bonds and 70% alpha(1->6) bonds. cf. EC 2.4.1.5, dextransucrase [5].
CAS REGISTRY NUMBER
COMMENTARY hide
81725-87-3
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
sucrose + (1,3-alpha-D-glucosyl)n
D-fructose + (1,3-alpha-D-glucosyl)n+1
show the reaction diagram
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-
-
-
?
sucrose + (1,6-alpha-D-glucosyl)n
D-fructose + (1,6-alpha-D-glucosyl)n+1
show the reaction diagram
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
6-Deoxysucrose
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competitive
6-Thiosucrose
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-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Dextran T10
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0071
Dextran
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pH 6.5, 37C
1.3 - 4.9
Sucrose
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.86 - 6.8
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-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
149000
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sedimentation equilibrium studies
151000
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1 * 151000, SDS-PAGE
159000
-
1 * 159000, SDS-PAGE
161000
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1 * 161000, enzyme I, SDS-PAGE
174000
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
2 isoenzymes: I and II; HS6, serotype a
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HS6, serotype a
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Ingbritt, serotype c, 3 isoenzymes
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in strain CH1 and CH107
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expression of mutants with different numbers of repeats in gtfG in strain CH1
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