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Literature summary for 2.4.1.125 extracted from

  • Tsumori, H.; Shimamura, A.; Mukasa, H.
    Purification and properties of extracellular glucosyltransferase synthesizing 1,6-, 1,3-alpha-D-glucan from Streptococcus mutans serotype a (1985), J. Gen. Microbiol., 131, 3347-3353.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Dextran T10 no stimulation in the range 0.01-2.0 mg/ml Streptococcus mutans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.9
-
sucrose pH 6.5, 37°C Streptococcus mutans

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Streptococcus mutans
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
159000
-
1 * 159000, SDS-PAGE Streptococcus mutans

Organism

Organism UniProt Comment Textmining
Streptococcus mutans
-
HS6, serotype a
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information 1.5% Streptococcus mutans

Purification (Commentary)

Purification (Comment) Organism
HS6, serotype a Streptococcus mutans

Source Tissue

Source Tissue Comment Organism Textmining
culture supernatant
-
Streptococcus mutans
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
89.7
-
-
Streptococcus mutans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose + (1,6-alpha-D-glucosyl)n also transfers glucosyl residues to the 3-position on glucose residues in glucans, producing a highly-branched 1,6-alpha-D-glucan Streptococcus mutans D-fructose + (1,6-alpha-D-glucosyl)n+1 glucan consists of 49.1 mol% 1,6-alpha-linked glucose and 33.9 mol% 1,3-alpha-linked glucose with 13.6 mol% terminal glucose and 3.3 mol% 1,3,6-alpha-branched glucose ?

Subunits

Subunits Comment Organism
monomer 1 * 159000, SDS-PAGE Streptococcus mutans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Streptococcus mutans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Streptococcus mutans