Information on EC 2.4.1.113 - alpha-1,4-glucan-protein synthase (ADP-forming)

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The expected taxonomic range for this enzyme is: Bacteria, Archaea

EC NUMBER
COMMENTARY hide
2.4.1.113
-
RECOMMENDED NAME
GeneOntology No.
alpha-1,4-glucan-protein synthase (ADP-forming)
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ADP-glucose + protein = ADP + alpha-D-glucosyl-protein
show the reaction diagram
The enzyme builds up alpha-1,4-glucan chains covalently bound to protein, thus acting as an initiator of glycogen synthesis.
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-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
ADP-glucose:protein 4-alpha-D-glucosyltransferase
The enzyme builds up alpha-1,4-glucan chains covalently bound to protein, thus acting as an initiator of glycogen synthesis.
CAS REGISTRY NUMBER
COMMENTARY hide
67053-99-0
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ADP-glucose + protein
ADP + alpha-D-glucosyl-protein
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ADP-glucose + protein
ADP + alpha-D-glucosyl-protein
show the reaction diagram
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Brij-58
-
-
Lubrol
-
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.9
ADP-glucose
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-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.2 - 7.5
-
half-maximal activity at pH 5.2 and pH 7.5
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
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assay at
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
70 - 90
-
55% activity at 70°C and 90°C
additional information
-
no activity at 25°C and 110°C
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
42000
-
2 * 42000, SDS-PAGE
85000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
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contains alpha-1,4-glucosidic bonds
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
80
-
stable for at least 2 h
90
-
half-life: 26 min, and 52 min in presence of 3.2 mM ADP-glucose
100
-
half-life: 3 min, and 9.5 min in presence of 3.2 mM ADP-glucose
110
-
inactivated, but in presence of 3.2 mM ADP-glucose the enzyme shows a half-life of 6 min
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
1% Brij-58 totally inhibited the enzyme activity of the stored enzyme preparation after 50 min
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, loss of about 90% activity after 30 days
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE