KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.9 | - |
ADP-glucose | - |
Thermococcus hydrothermalis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
2 * 42000, SDS-PAGE | Thermococcus hydrothermalis |
85000 | - |
gel filtration | Thermococcus hydrothermalis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP-glucose + protein | Thermococcus hydrothermalis | - |
ADP + alpha-D-glucosyl-protein | - |
? | |
ADP-glucose + protein | Thermococcus hydrothermalis AL662 | - |
ADP + alpha-D-glucosyl-protein | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus hydrothermalis | - |
AL662 | - |
Thermococcus hydrothermalis AL662 | - |
AL662 | - |
Purification (Comment) | Organism |
---|---|
partial | Thermococcus hydrothermalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP-glucose + protein | - |
Thermococcus hydrothermalis | ADP + alpha-D-glucosyl-protein | - |
? | |
ADP-glucose + protein | best donor substrate, enzyme can also use UDP-glucose as donor substrate | Thermococcus hydrothermalis | ADP + alpha-D-glucosyl-protein | - |
? | |
ADP-glucose + protein | - |
Thermococcus hydrothermalis AL662 | ADP + alpha-D-glucosyl-protein | - |
? | |
ADP-glucose + protein | best donor substrate, enzyme can also use UDP-glucose as donor substrate | Thermococcus hydrothermalis AL662 | ADP + alpha-D-glucosyl-protein | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 42000, SDS-PAGE | Thermococcus hydrothermalis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
- |
Thermococcus hydrothermalis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
no activity at 25°C and 110°C | Thermococcus hydrothermalis |
70 | 90 | 55% activity at 70°C and 90°C | Thermococcus hydrothermalis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
stable for at least 2 h | Thermococcus hydrothermalis |
90 | - |
half-life: 26 min, and 52 min in presence of 3.2 mM ADP-glucose | Thermococcus hydrothermalis |
100 | - |
half-life: 3 min, and 9.5 min in presence of 3.2 mM ADP-glucose | Thermococcus hydrothermalis |
110 | - |
inactivated, but in presence of 3.2 mM ADP-glucose the enzyme shows a half-life of 6 min | Thermococcus hydrothermalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Thermococcus hydrothermalis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.2 | 7.5 | half-maximal activity at pH 5.2 and pH 7.5 | Thermococcus hydrothermalis |