Information on EC 2.3.1.80 - cysteine-S-conjugate N-acetyltransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.3.1.80
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RECOMMENDED NAME
GeneOntology No.
cysteine-S-conjugate N-acetyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
acetyl-CoA + an L-cysteine-S-conjugate = CoA + an N-acetyl-L-cysteine-S-conjugate
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acyl group transfer
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
4-hydroxy-2-nonenal detoxification
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glutathione-mediated detoxification I
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Glutathione metabolism
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SYSTEMATIC NAME
IUBMB Comments
acetyl-CoA:S-substituted L-cysteine N-acetyltransferase
S-Benzyl-L-cysteine and, in decreasing order of activity, S-butyl-L-cysteine, S-propyl-L-cysteine, O-benzyl-L-serine and S-ethyl-L-cysteine, can act as acceptors.
CAS REGISTRY NUMBER
COMMENTARY hide
81725-80-6
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + an S-substituted L-cysteine
CoA + an S-substituted N-acetyl-L-cysteine
show the reaction diagram
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final reaction of mercapturic biosynthesis
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acetyl-CoA + leukotriene E4
CoA + N-acetyl-leukotriene E4
show the reaction diagram
about 1% of the activity with S-benzyl-L-cysteine
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-
?
acetyl-CoA + O-benzyl-L-serine
CoA + N-acetyl-O-benzyl-L-cysteine
show the reaction diagram
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-
-
-
?
acetyl-CoA + S-4-nitrobenzyl-L-cysteine
CoA + ?
show the reaction diagram
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-
-
-
?
acetyl-CoA + S-benzyl-L-cysteine
CoA + N-acetyl-S-benzyl-L-cysteine
show the reaction diagram
acetyl-CoA + S-butyl-L-cysteine
CoA + N-acetyl-S-butyl-L-cysteine
show the reaction diagram
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-
-
-
?
acetyl-CoA + S-ethyl-L-cysteine
CoA + N-acetyl-S-ethyl-L-cysteine
show the reaction diagram
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-
-
-
?
acetyl-CoA + S-propyl-L-cysteine
CoA + N-acetyl-S-propyl-L-cysteine
show the reaction diagram
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-
-
-
?
S-(1,2,3,4,4-pentachloro-butadienyl)-L-cysteine + acetyl-CoA
N-acetyl-S-(1,2,3,4,4-pentachlorobutadienyl)-L-cysteine + CoA
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + an S-substituted L-cysteine
CoA + an S-substituted N-acetyl-L-cysteine
show the reaction diagram
-
final reaction of mercapturic biosynthesis
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5,5'-dithiobis-2-nitrobenzoic acid
strong inhibition
N-ethylmaleimide
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octyl glucoside
10 mM, 80% residual activity. Slight activation at 1 mM
p-chloromercuribenzoate
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probenecid
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less pronounced inhibition
additional information
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not inhibitory: PMSF
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
octyl glucoside
1 mM, slight stimulation, inhibitory at higher concentration
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.023
acetyl-CoA
pH 7.5, 37°C
2.6
O-benzyl-L-serine
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0.19 - 0.39
S-(1,2,3,4,4-pentachloro-butadienyl)-L-cysteine
0.176
S-4-nitrobenzyl-L-cysteine
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0.026
S-acetyl-CoA
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0.064 - 0.14
S-Benzyl-L-cysteine
0.063
S-butyl-L-cysteine
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7.1
S-ethyl-L-cysteine
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0.67
S-propyl-L-cysteine
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additional information
additional information
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
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assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
32000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 29000, SDS-PAGE of recombinant protein including His-tag
monomer
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1 * 34000, SDS-PAGE
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20 C, stable for several months
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4°C, glycine/deoxyBIGCHAP buffer, pH 7.0, considerable inactivation
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
amino acid analysis
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in HEK-293T cell
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E104K
frequently found single nucleotide polymorphism, does not change enzymic activity or the expression of protein by more than 2fold
F143S
frequently found single nucleotide polymorphism, does not change enzymic activity or the expression of protein by more than 2fold
R149K
suppression of activity
additional information
a truncated protein starting from residue M25 shows no cysteinyl-S-conjugate N-acetyltransferase activity
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
the frequently found single nucleotide polymorphisms, E104K or F143S, do not change enzymic activity or the expression of protein by more than 2fold