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Literature summary for 2.3.1.80 extracted from

  • Veiga-da-Cunha, M.; Tyteca, D.; Stroobant, V.; Courtoy, P.J.; Opperdoes, F.R.; Van Schaftingen, E.
    Molecular identification of NAT8 as the enzyme that acetylates cysteine S-conjugates to mercapturic acids (2010), J. Biol. Chem., 285, 18888-18898.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.80

Activating Compound

Activating Compound Comment Organism Structure
octyl glucoside 1 mM, slight stimulation, inhibitory at higher concentration Homo sapiens

Application

Application Comment Organism
medicine the frequently found single nucleotide polymorphisms, E104K or F143S, do not change enzymic activity or the expression of protein by more than 2fold Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression in HEK-293T cell Homo sapiens

Protein Variants

Protein Variants Comment Organism
E104K frequently found single nucleotide polymorphism, does not change enzymic activity or the expression of protein by more than 2fold Homo sapiens
F143S frequently found single nucleotide polymorphism, does not change enzymic activity or the expression of protein by more than 2fold Homo sapiens
additional information a truncated protein starting from residue M25 shows no cysteinyl-S-conjugate N-acetyltransferase activity Homo sapiens
R149K suppression of activity Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis-2-nitrobenzoic acid strong inhibition Homo sapiens
octyl glucoside 10 mM, 80% residual activity. Slight activation at 1 mM Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.023
-
 acetyl-CoA pH 7.5, 37°C Homo sapiens
0.064
-
 S-Benzyl-L-cysteine pH 7.5, 37°C Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
 x * 29000, SDS-PAGE of recombinant protein including His-tag Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9UHE5
-
-

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
 Homo sapiens
-
liver
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + leukotriene E4 about 1% of the activity with S-benzyl-L-cysteine Homo sapiens CoA + N-acetyl-leukotriene E4
-
 ?
acetyl-CoA + S-benzyl-L-cysteine
-
 Homo sapiens CoA + N-acetyl-S-benzyl-L-cysteine
-
 ?
additional information besides S-benzyl-L-cysteine and leukotriene E4, enzyme is inactive on physiological amines or amino acids L-aspartate, L-lysine, L-cystine, L-methionine, L-ethionine, L-cystathionine, or L-phenylalanine, L-tyrosine, and L-tryptophan Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
? x * 29000, SDS-PAGE of recombinant protein including His-tag Homo sapiens

Synonyms

Synonyms Comment Organism
NAT8
-
 Homo sapiens