Information on EC 2.3.1.192 - glycine N-phenylacetyltransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.3.1.192
-
RECOMMENDED NAME
GeneOntology No.
glycine N-phenylacetyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
phenylacetyl-CoA + glycine = CoA + phenylacetylglycine
show the reaction diagram
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
L-phenylalanine degradation IV (mammalian, via side chain)
-
-
Phenylalanine metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
phenylacetyl-CoA:glycine N-phenylacetyltransferase
Not identical with EC 2.3.1.13 (glycine N-acyltransferase). This enzyme was purified from bovine liver mitochondria. L-asparagine, L-glutamine and L-arginine are alternative substrates to glycine, but have higher Km values.
CAS REGISTRY NUMBER
COMMENTARY hide
71567-08-3
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1-naphthylacetyl-CoA + glycine
1-naphthylacetylglycine + CoA
show the reaction diagram
-
best substrate
-
-
?
1-naphtylacetyl-CoA + L-glutamine
? + CoA
show the reaction diagram
-
3-5% of the rate with phenacetyl-CoA
-
-
?
2,4-dichlorophenoxyacetyl-CoA + glycine
2,4-dichlorophenoxyacetylglycine + CoA
show the reaction diagram
-
-
-
-
?
indoleacetyl-CoA + glycine
N-indoleacetylglycine + CoA
show the reaction diagram
-
-
-
-
?
indoleacetyl-CoA + L-glutamine
? + CoA
show the reaction diagram
-
3-5% of the rate with phenacetyl-CoA
-
-
?
phenoxyacetyl-CoA + glycine
phenoxyacetylglycine + CoA
show the reaction diagram
-
overall rate of conjugation of the phenoxyherbicides are slow relative to the standard substrate phenylacetyl-CoA
-
-
?
phenylacetyl-CoA + glycine
phenylacetylglycine + CoA
show the reaction diagram
phenylacetyl-CoA + L-arginine
? + CoA
show the reaction diagram
-
at 20% of the rate with glycine
-
-
?
phenylacetyl-CoA + L-asparagine
? + CoA
show the reaction diagram
-
stereospecific for the L-isomer of asparagine
-
-
?
phenylacetyl-CoA + L-glutamine
? + CoA
show the reaction diagram
phenylacetyl-CoA + L-glutamine
phenylacetyl-L-glutamine + CoA
show the reaction diagram
-
may substitue for glycine, but at lower rate
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
activation at both physiological and high substrate concentration
K2SO4
-
55 mM, 67% residual activity at physiological substrate concentration, 110% of initial activity at high substrate concentration
Mg2+
-
1 mM, about 10% activation, 10 mM, inihibition at physiological substrate concentration, activation at high substrate concentration
additional information
-
addition of monovalent cations is required
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,4,5-trichlorophenoxyacetyl-CoA
-
competitive
2,4-dichlorophenoxyacetyl-CoA
-
competitive
5,5'-dithiobis(2-nitrobenzoate)
-
phenylacetyl-CoA partially protects phenylacetyltransferase against 5,5'-dithiobis(2-nitrobenzoate) inactivation
acylglycine
-
noncompetitive
benzoyl-CoA
-
-
butyryl-CoA
-
-
citrate
-
40 mM, 22% residual activity
K2SO4
-
55 mM, 67% residual activity at physiological substrate concentration, 110% of initial activity at high substrate concentration
Mg2+
-
1 mM, about 10% activation, 10 mM, inihibition at physiological substrate concentration, activation at high substrate concnetration
Ni2+
-
2 mM, 28% residual activity
p-chloromercuribenzoate
-
-
Zn2+
-
1 mM, 46% residual activity
additional information
-
human enzyme is insensitive to salts
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KCl
-
110 mM, 72% residual activity at physiologic substrate concentration, 195% of initial activity at high substrate concentration
phosphate
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37 mM, 140-175% of initial activity at low and high substrate concentration
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
9 - 1000
glycine
120
L-glutamine
-
pH 8.0, 30°C
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.1
CoA
Bos taurus
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pH 8.0, 30°C, assay at 3.5 mM glycine, 0.020 mM phenylacetyl-CoA and in the absence of ions
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.051
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pH not specified in the publication, temperature not specified in the publication
49
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pH 8.0, 30°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.4 - 8.6
-
-
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
-
isoelectric focusing
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
32000
-
gel filtration
33000
-
gel filtration
33500
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1 * 33500, SDS-PAGE
38937
-
x * 38937, calculated
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 38937, calculated
monomer
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
proteolytic modification
-
presence of a signal peptide of 5 kDa
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE