Activating Compound | Comment | Organism | Structure |
---|---|---|---|
KCl | 110 mM, 72% residual activity at physiologic substrate concentration, 195% of initial activity at high substrate concentration | Bos taurus | |
phosphate | 37 mM, 140-175% of initial activity at low and high substrate concentration | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
citrate | 40 mM, 22% residual activity | Bos taurus | |
CoA | at physiologic concentrations of substrate, the arylacetyl transferase is extensively inhibited by CoA, inhibition is greatly reduced by ions. The 3-phosphate group on CoA is important for binding to the salt-free enzyme but in the presence of ions its importance is diminished | Bos taurus | |
K2SO4 | 55 mM, 67% residual activity at physiological substrate concentration, 110% of initial activity at high substrate concentration | Bos taurus | |
KCl | 110 mM, 72% residual activity at physiologic substrate concentration, 195% of initial activity at high substrate concentration. Inhibition results in a large decrease in the affinity of the enzyme for phenylacetyl-CoA. In the presence of KCl the KD values for phenylacetyl-CoA and naphthylacetyl-CoA are similar, but the KD for glycine is extremely high for 1-naphthylacetyl-CoA conjugation | Bos taurus | |
Mg2+ | 1 mM, about 10% activation, 10 mM, inihibition at physiological substrate concentration, activation at high substrate concnetration | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Bos taurus | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activation at both physiological and high substrate concentration | Bos taurus | |
K2SO4 | 55 mM, 67% residual activity at physiological substrate concentration, 110% of initial activity at high substrate concentration | Bos taurus | |
Mg2+ | 1 mM, about 10% activation, 10 mM, inihibition at physiological substrate concentration, activation at high substrate concentration | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
gel filtration | Bos taurus |
33500 | - |
1 * 33500, SDS-PAGE | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
49 | - |
pH 8.0, 30°C | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-naphthylacetyl-CoA + glycine | best substrate | Bos taurus | 1-naphthylacetylglycine + CoA | - |
? | |
additional information | arginine and glutamine can substitute for glycine in the phenylacetyl-CoA assay and, while the rates are lower, they are equivalently affected by salt. No substrate: benzoyl-CoA, butyryl-CoA, and salicyl-CoA | Bos taurus | ? | - |
? | |
phenylacetyl-CoA + glycine | best substrate | Bos taurus | phenylacetylglycine + CoA | - |
? | |
phenylacetyl-CoA + L-arginine | at 20% of the rate with glycine | Bos taurus | ? + CoA | - |
? | |
phenylacetyl-CoA + L-glutamine | at 6% of the rate with glycine | Bos taurus | ? + CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 33500, SDS-PAGE | Bos taurus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Bos taurus | isoelectric focusing | - |
7.5 |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.1 | - |
pH 8.0, 30°C, assay at 3.5 mM glycine, 0.020 mM phenylacetyl-CoA and in the absence of ions | Bos taurus | CoA |