Information on EC 2.1.1.323 - (-)-pluviatolide 4-O-methyltransferase

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The expected taxonomic range for this enzyme is: Sinopodophyllum hexandrum

EC NUMBER
COMMENTARY hide
2.1.1.323
-
RECOMMENDED NAME
GeneOntology No.
(-)-pluviatolide 4-O-methyltransferase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + (-)-pluviatolide = S-adenosyl-L-homocysteine + (-)-bursehernin
show the reaction diagram
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
yatein biosynthesis I
-
-
SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:(-)-pluviatolide 4-O-methyltransferase
The enzyme, characterized from the plant Sinopodophyllum hexandrum, is involved in the biosynthetic pathway of podophyllotoxin, a non-alkaloid toxin lignan whose derivatives are important anticancer drugs.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
mayapple
UniProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + (-)-arctigenin
S-adenosyl-L-homocysteine + ?
show the reaction diagram
low efficiency substrate
-
-
?
S-adenosyl-L-methionine + (-)-matairesinol
S-adenosyl-L-homocysteine + ?
show the reaction diagram
low efficiency substrate
-
-
?
S-adenosyl-L-methionine + (-)-pluviatolide
S-adenosyl-L-homocysteine + (-)-bursehernin
show the reaction diagram
-
-
-
?
additional information
?
-
the enzyme cannot turn over (+)-pinoresinol
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + (-)-pluviatolide
S-adenosyl-L-homocysteine + (-)-bursehernin
show the reaction diagram
A0A0N9HMN6
-
-
-
?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0014
(-)-pluviatolide
pH and temperature not specified in the publication
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.72
(-)-pluviatolide
Sinopodophyllum hexandrum
A0A0N9HMN6
pH and temperature not specified in the publication
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Nicotiana tabacum leaves and Escherichia coli
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
enzyme expression is strongly increased 9 h after leaf wounding