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Literature summary for 2.1.1.323 extracted from

  • Lau, W.; Sattely, E.
    Six enzymes from mayapple that complete the biosynthetic pathway to the etoposide aglycone (2015), Science, 349, 1224-1228.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.323

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Nicotiana tabacum leaves and Escherichia coli Sinopodophyllum hexandrum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0014
-
 (-)-pluviatolide pH and temperature not specified in the publication Sinopodophyllum hexandrum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + (-)-pluviatolide Sinopodophyllum hexandrum
-
 S-adenosyl-L-homocysteine + (-)-bursehernin
-
 ?

Organism

Organism UniProt Comment Textmining
Sinopodophyllum hexandrum A0A0N9HMN6 mayapple
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme cannot turn over (+)-pinoresinol Sinopodophyllum hexandrum ?
-
 ?
S-adenosyl-L-methionine + (-)-arctigenin low efficiency substrate Sinopodophyllum hexandrum S-adenosyl-L-homocysteine + ?
-
 ?
S-adenosyl-L-methionine + (-)-matairesinol low efficiency substrate Sinopodophyllum hexandrum S-adenosyl-L-homocysteine + ?
-
 ?
S-adenosyl-L-methionine + (-)-pluviatolide
-
 Sinopodophyllum hexandrum S-adenosyl-L-homocysteine + (-)-bursehernin
-
 ?

Synonyms

Synonyms Comment Organism
OMT3
-
 Sinopodophyllum hexandrum
pluviatolide 4-O-methyltransferase
-
 Sinopodophyllum hexandrum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.72
-
 (-)-pluviatolide pH and temperature not specified in the publication Sinopodophyllum hexandrum

Expression

Organism Comment Expression
Sinopodophyllum hexandrum enzyme expression is strongly increased 9 h after leaf wounding up