Information on EC 2.1.1.10 - homocysteine S-methyltransferase

Word Map on EC 2.1.1.10
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
2.1.1.10
-
RECOMMENDED NAME
GeneOntology No.
homocysteine S-methyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S-methyl-L-methionine + L-homocysteine = 2 L-methionine
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
methyl group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
L-methionine biosynthesis II
-
-
L-methionine salvage from L-homocysteine
-
-
S-methyl-L-methionine cycle
-
-
methionine metabolism
-
-
Cysteine and methionine metabolism
-
-
Metabolic pathways
-
-
Biosynthesis of secondary metabolites
-
-
SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:L-homocysteine S-methyltransferase
The enzyme uses S-adenosyl-L-methionine as methyl donor less actively than S-methyl-L-methionine.
CAS REGISTRY NUMBER
COMMENTARY hide
9012-40-2
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
var. italica, broccoli
TrEMBL
Manually annotated by BRENDA team
strain ATCC 9950
-
-
Manually annotated by BRENDA team
strain NRRL 199
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain Texas M
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
-
prevents accumulation of (R,S)-S-adenosyl methionine (formed by spontaneous racemization)
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(R,S)-S-adenosyl-L-methionine + L-homocysteine
L-methionine + S-adenosyl-L-homocysteine
show the reaction diagram
(S,S)-S-adenosyl-L-methionine + L-homocysteine
L-methionine + S-adenosyl-L-homocysteine
show the reaction diagram
-
higher affinity for R,S form
-
-
?
S-adenosyl-D-methionine + L-homocysteine
?
show the reaction diagram
S-adenosyl-L-ethionine + L-homocysteine
S-adenosyl-L-homocysteine + L-ethionine
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + D-homocysteine
?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + DL-cysteine
S-adenosyl-L-homocysteine + S-methyl-DL-cysteine
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + DL-homocysteine
L-methionine + S-adenosyl-L-homocysteine
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + DL-selenocysteine
S-adenosyl-L-homocysteine + Se-methyl-DL-selenocysteine
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + L-cysteine
S-adenosyl-L-homocysteine + S-methyl-L-cysteine
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + L-homocysteine
?
show the reaction diagram
-
low activity
-
-
?
S-adenosyl-L-methionine + L-homocysteine
S-adenosyl-L-homocysteine + L-methionine
show the reaction diagram
S-adenosyl-L-methionine + L-homocysteine thiolactone
?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + L-selenocysteine
S-adenosyl-L-homocysteine + Se-methyl-L-selenocysteine
show the reaction diagram
-
-
-
?
S-methyl-L-methionine + L-cysteine
?
show the reaction diagram
-
-
-
?
S-methyl-L-methionine + L-homocysteine
2 L-methionine
show the reaction diagram
S-methyl-L-methionine + L-homocysteine
?
show the reaction diagram
S-methyl-L-methionine + L-homocysteine
L-methionine
show the reaction diagram
S-methyl-L-methionine + selenohomocysteine
?
show the reaction diagram
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + L-homocysteine
S-adenosyl-L-homocysteine + L-methionine
show the reaction diagram
S-methyl-L-methionine + L-homocysteine
2 L-methionine
show the reaction diagram
S-methyl-L-methionine + L-homocysteine
L-methionine
show the reaction diagram
Q47690
metabolism of S-methylmethionine requires a functional YagD protein, since inactivation of the yagD gene abolishes the capacity to grow on S-methylmethionine
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
L-methionine
N-Hydroxymethyl-DL-methionine
-
-
S-adenosyl-L-homocysteine
S-adenosyl-L-methionine
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
-
increases activity at low substrate concentrations
cysteine
-
-
homocysteine
-
induces reaction
homoserine
-
-
S-[(2R)-2-amino-2-carboxyethyl]-L-homocysteine
-
-
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.072
DL-homocysteine
-
0.018 - 0.077
L-homocysteine
0.225 - 1.95
S-adenosyl-L-methionine
0.029 - 0.94
S-methyl-L-methionine
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0375
DL-homocysteine
Brassica oleracea
A4ZGQ8
-
0.38
L-homocysteine
Escherichia coli
-
pH 6, 30C, cosubstrate: S-adenosyl-L-methionine
additional information
additional information
Homo sapiens
-
similar turnover number like betaine-homocysteine methyltransferase (BHMT, EC 2.1.1.5)
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0008
-
kidney, S-methyl-L-methionine as methyl donor
0.0009
using L-selenocysteine as substrate
0.0017
using DL-selenocysteine as substrate
0.0022
-
liver, S-adenosyl-L-methionine as methyl donor
0.0054
using L-cysteine as substrate
0.0087
-
liver, S-methyl-L-methionine as methyl donor
0.012
-
S-methyl-L-methionine as methyl donor
0.014
-
S-methyl-L-methionine as methyl donor
0.0147
using DL-cysteine as substrate
0.017
-
S-methyl-L-methionine as methyl donor
0.0936
using DL-homocysteine as substrate
1.37
-
purified enzyme, S-adenosyl-L-methionine as methyl donor
additional information
-
structural comparison and enzymatic properties of purified human betaine-homocysteine methyltransferase (BHMT, EC 2.1.1.5) and human betaine-homocysteine methyltransferase-2 (BHMT-2) determined, methylation capacities of homocysteine analyzed, S-adenosyl-L-methionine-dependent methylation of homocysteine predicted mostly occurs via BHMT-2 in vivo
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 7
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
-
activity assay at
30 - 35
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
rosette leaves, cauline leaves
Manually annotated by BRENDA team
additional information
-
no activity detected in brain and heart
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30000
-
from sedimentation coefficient
34000
-
gel filtration
35000
-
gel filtration
35400
calculated from amino acid sequence
36000
gel filtration; gel filtration
40000
-
SDS-PAGE, 73% sequence identity with the 45 kDa BHMT protein (EC 2.1.1.5)
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
in oxidized, apo, and metallated form, to 1.8-1.9 A resolution
-
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
high concentrations of glycerol (40%, v/v) greatly stabilized activity
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gel filtration, recombinant protein
-
recombinant enzyme
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
expressed in Escherichia coli, recombinant protein, intein/chitin-binding fusion constructs, pTBY3 vector
-
expression in Escherichia coli
expression in Escherichia coli; expression in Escherichia coli
in Escherichia coli
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Y71F/T169Y
-
mutant designed to mimic the betaine-homocysteine methyltransferase substrate binding pocket, complete loss of activity
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
-
determination of total homocysteine in plasma and serum
Show AA Sequence (1263 entries)
Please use the Sequence Search for a certain query.