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Literature summary for 2.1.1.10 extracted from

  • Szegedi, S.S.; Castro, C.C.; Koutmos, M.; Garrow, T.A.
    Betaine-homocysteine S-methyltransferase-2 is an S-methylmethionine-homocysteine methyltransferase (2008), J. Biol. Chem., 283, 8939-8945.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.10

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli, recombinant protein, intein/chitin-binding fusion constructs, pTBY3 vector Homo sapiens

General Stability

General Stability Organism
high concentrations of glycerol (40%, v/v) greatly stabilized activity Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
L-methionine stronger inhibitor of BHMT-2 than of BHMT (EC 2.1.1.5) Homo sapiens
additional information relative inhibition of human BHMT (EC 2.1.1.5) and human BHMT-2 activities by methyl donor substrates determined Homo sapiens
S-adenosyl-L-methionine weak inhibitor of, does not inhibit BHMT (EC 2.1.1.5), not inhibited by betaine Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.94
-
 S-methyl-L-methionine final concentrations of S-methyl-L-methionine ranging from 0.1 mM to 9 mM, lower value than obtained for BHMT (EC 2.1.1.5) Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ required for activity Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 SDS-PAGE, 73% sequence identity with the 45 kDa BHMT protein (EC 2.1.1.5) Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
gel filtration, recombinant protein Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
S-methyl-L-methionine + L-homocysteine = 2 L-methionine high homology to betaine-homocysteine methyltransferase (BHMT, EC 2.1.1.5), betaine is not a substrate for BHMT-2 but high activity with S-methyl-L-methionine Homo sapiens
a

Source Tissue

Source Tissue Comment Organism Textmining
kidney tissue extracts, activities measured in Homo sapiens
-
liver tissue extracts, activities measured in Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 structural comparison and enzymatic properties of purified human betaine-homocysteine methyltransferase (BHMT, EC 2.1.1.5) and human betaine-homocysteine methyltransferase-2 (BHMT-2) determined, methylation capacities of homocysteine analyzed, S-adenosyl-L-methionine-dependent methylation of homocysteine predicted mostly occurs via BHMT-2 in vivo Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + L-homocysteine low activity Homo sapiens ?
-
 ?
S-methyl-L-methionine + L-homocysteine high homology to betaine-homocysteine methyltransferase (BHMT, EC 2.1.1.5), betaine is not a substrate for BHMT-2 but high activity with S-methyl-L-methionine Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
BHMT-2
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
7.5
-
 activity assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information similar turnover number like betaine-homocysteine methyltransferase (BHMT, EC 2.1.1.5) Homo sapiens