Information on EC 1.3.1.16 - beta-nitroacrylate reductase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.3.1.16
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RECOMMENDED NAME
GeneOntology No.
beta-nitroacrylate reductase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
3-nitropropanoate + NADP+ = 3-nitroacrylate + NADPH + H+
show the reaction diagram
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-
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-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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-
-
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redox reaction
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-
-
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reduction
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-
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SYSTEMATIC NAME
IUBMB Comments
3-nitropropanoate:NADP+ oxidoreductase
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CAS REGISTRY NUMBER
COMMENTARY hide
37255-28-0
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
baker´s yeast, oriental yeast Japan
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(Z)-3-phenyl-2-nitro-2-butene + NADPH
(2R,3R)-3-phenyl-2-nitrobutane + (2S,3R)-3-phenyl-2-nitrobutane + NADP+
show the reaction diagram
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excellent enantioselectivity, moderate diastereoselectivity, mechanism
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-
?
10-nitrooctadec-9-enoic acid + NADPH + H+
10-nitrostearic acid + NADP+
show the reaction diagram
3-nitroacrylate + NADPH
3-nitropropanoate + NADP+
show the reaction diagram
9-nitrooctandec-9-enoic acid + NADPH + H+
9-nitrostearic acid + NADP+
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3-nitroacrylate + NADPH
3-nitropropanoate + NADP+
show the reaction diagram
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biosynthesis of beta-nitropropionic acid
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-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADPH
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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enzyme is inhibited by sulfhydryl compounds, e.g. cysteine at concentrations equivalent to 3-nitroacrylic acid, inhibition probably due to the reaction of the sulfhydryl compounds with 3-nitroacrylic acid rather than an inhibition of the enzyme itself
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.000085
(9E)-9-nitrooctadec-9-enoic acid
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pH 7.0, temperature not specified in the publication
0.16
3-Nitroacrylate
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
81
(9E)-9-nitrooctadec-9-enoic acid
Homo sapiens
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pH 7.0, temperature not specified in the publication
90004
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3 - 7
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at pH 3 about 35% of activity maximum, at pH 7 about 60% of activity maximum
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in HEK-293T cell