Information on EC 1.1.99.21 - D-sorbitol dehydrogenase (acceptor)

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Gluconobacter

EC NUMBER
COMMENTARY hide
1.1.99.21
-
RECOMMENDED NAME
GeneOntology No.
D-sorbitol dehydrogenase (acceptor)
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
D-sorbitol + acceptor = L-sorbose + reduced acceptor
show the reaction diagram
; a flavoprotein, FAD
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
2-keto-L-gulonate biosynthesis
-
-
Fructose and mannose metabolism
-
-
L-ascorbate biosynthesis III
-
-
ascorbate metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
D-sorbitol:acceptor 1-oxidoreductase
A flavoprotein (FAD).
CAS REGISTRY NUMBER
COMMENTARY hide
86178-94-1
not distinguished from EC 1.1.1.15 in Chemical Abstracts
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain M5
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1-(2-hydroxyethyl)amino-1-deoxy-D-sorbitol + acceptor
6-(2-hydroxyethyl)amino-6-deoxy-L-sorbose + reduced acceptor
show the reaction diagram
1-benzyloxycarbonylamino-1-deoxy-D-sorbitol + acceptor
N-benzyloxycarbonyl-6-amino-L-sorbose + reduced acceptor
show the reaction diagram
-
-
-
-
-
D-arabitol + acceptor
D-xylulose + reduced acceptor
show the reaction diagram
-
-
-
-
?
D-gluconate + acceptor
5-keto-D-gluconate + reduced acceptor
show the reaction diagram
-
-
-
-
?
D-mannitol + acceptor
? + reduced acceptor
show the reaction diagram
-
oxidation at 5% the rate of D-sorbitol
-
-
?
D-mannitol + acceptor
D-fructose + reduced acceptor
show the reaction diagram
-
-
-
-
?
D-sorbitol + acceptor
L-sorbose + reduced acceptor
show the reaction diagram
D-sorbitol + acceptor
sorbose + reduced acceptor
show the reaction diagram
D-sorbitol + phenazine methosulfate
?
show the reaction diagram
-
the catalytic reaction follows an ordered Bi Bi mechanism, the native mSLDH bears two different substrate-binding sites, one for ubiquinone using as electron acceptor and the other for D-sorbitol, in addition to PQQ-binding and Mg2+-binding sites in the catalytic center
-
-
?
D-sorbitol + ubiquinone-2
?
show the reaction diagram
-
-
-
-
?
glycerol + acceptor
dihydroxyacetone + reduced acceptor
show the reaction diagram
-
-
-
-
?
meso-erythritol + acceptor
L-erythrulose + reduced acceptor
show the reaction diagram
-
-
-
-
?
ribitol + acceptor
? + reduced acceptor
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
D-sorbitol + acceptor
L-sorbose + reduced acceptor
show the reaction diagram
additional information
?
-
-
production of 5-keto-D-gluconate is solely dependent on enzyme
-
-
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,6-dichlorophenolindophenol
-
acts in vitro as acceptor together with phenazine methosulfate
cytochrome c
additional information
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
KCl
-
activation
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
D-sorbose
-
non-competitive inhibition, sorbitol or phenazine methosulfate as substrates
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
16.8 - 30
D-sorbitol
0.0314
phenazine methosulfate
-
-
0.0257
ubiquinone-2
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
9
-
about 50% of activity at pH 5.0
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
17000
-
1 * 63000 + 1 * 51000 + 1 * 17000, dissociation into 3 components, a flavoprotein (MW 63000), a cytochrome c (MW 51000) and an unknown polypeptide (MW 17000), SDS-PAGE
51000
-
1 * 63000 + 1 * 51000 + 1 * 17000, dissociation into 3 components, a flavoprotein (MW 63000), a cytochrome c (MW 51000) and an unknown polypeptide (MW 17000), SDS-PAGE
63000
-
1 * 63000 + 1 * 51000 + 1 * 17000, dissociation into 3 components, a flavoprotein (MW 63000), a cytochrome c (MW 51000) and an unknown polypeptide (MW 17000), SDS-PAGE
80000
-
x * 80000 (subunit SldA, calculated: 81730 Da), SDS-PAGE
131000
-
calculated sum of each MW of the 3 subunits
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
1 * 63000 + 1 * 51000 + 1 * 17000, dissociation into 3 components, a flavoprotein (MW 63000), a cytochrome c (MW 51000) and an unknown polypeptide (MW 17000), SDS-PAGE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
D-sorbitol stabilizes during solubilization and purification
-
ionic detergents, such as cetylpyridinium chloride, cetyltrimethyl ammonium bromide and sodium stearate, inactivate
-
Triton X-100, best detergent for solubilization
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
5°C, 50% loss of activity overnight
-
5°C, storage for several months leads to cytochrome c decomposition
-
stable at 80°C for more than two months in the presence of 0.1% Tween-20
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
by cell disruption and ultracentrifugation
-
by CM-cellulose and sephacryl HR 400 column chromatography
-
ion exchange chromatography, gel filtration
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
in Escherichia coli, plasmids harboring the disrupted gene are introduced into Gluconobacter frateurii THD32 by electroporation
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
transcriptional regulator SldR induces the expression of the enzyme after L-sorbose has accumulated
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
enzyme disruption mutant, oxidation activity against D-arabitol, D-sorbitol, D-mannitol, ribitol, meso-erythritol and glycerol are diminished
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
-
production of 6-amino-L-sorbose, which is an intermediate in the miglitol production and an intermediate for oral alpha-glucosidase-inhibitors
Show AA Sequence (294 entries)
Please use the Sequence Search for a specific query.