Information on EC 1.1.1.65 - pyridoxine 4-dehydrogenase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.1.1.65
-
RECOMMENDED NAME
GeneOntology No.
pyridoxine 4-dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
pyridoxine + NADP+ = pyridoxal + NADPH + H+
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
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redox reaction
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-
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reduction
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis (yeast)
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Metabolic pathways
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pyridoxal 5'-phosphate salvage II (plants)
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Vitamin B6 metabolism
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SYSTEMATIC NAME
IUBMB Comments
pyridoxine:NADP+ 4-oxidoreductase
Also oxidizes pyridoxine phosphate.
CAS REGISTRY NUMBER
COMMENTARY hide
9028-64-2
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-nitrobenzaldehyde + NADPH
2-nitrobenzyl alcohol + NADP+
show the reaction diagram
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-
-
?
pyridoxine + NADP+
pyridoxal + NADPH
show the reaction diagram
pyridoxine phosphate + NADP+
pyridoxal 5'-phosphate + NADPH
show the reaction diagram
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
pyridoxine + NADP+
pyridoxal + NADPH
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ZnCl2
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0.1 mM: activation, 10 mM: inhibition
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
p-Chloromercuriphenyl sulfonic acid
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ZnCl2
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0.1 mM: activation, 10 mM: inhibition
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.096
2-nitrobenzaldehyde
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0.9 - 1.6
pyridoxal
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 7.5
for formation of pyridoxine
7.5 - 8.5
for formation of pyridoxal
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 8
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at pH 5.0 and 8.0 about 50% of activity maximum
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
41000
gel filtration
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
purified enzyme very unstable but can be stabilized by low concentrations of various detergents such as Tween 40
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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