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Literature summary for 1.1.1.65 extracted from

  • Holzer, H.; Schneider, S.
    Purification and characterization of a TPN-dependent pyridoxol dehydrogenase from brewers yeast (1961), Biochim. Biophys. Acta, 48, 71-76.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
CoCl2
-
Saccharomyces cerevisiae
CuSO4
-
Saccharomyces cerevisiae
MnCl2
-
Saccharomyces cerevisiae
p-chloromercuriphenyl sulfonic acid
-
Saccharomyces cerevisiae
SO32-
-
Saccharomyces cerevisiae
ZnCl2 0.1 mM: activation, 10 mM: inhibition Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
NADPH
-
Saccharomyces cerevisiae
0.02
-
NADP+
-
Saccharomyces cerevisiae
1.6
-
pyridoxal
-
Saccharomyces cerevisiae
7
-
pyridoxol
-
Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
ZnCl2 0.1 mM: activation, 10 mM: inhibition Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pyridoxine + NADP+ Saccharomyces cerevisiae pyridoxine is identical with pyridoxol, equilibrium lies far to the side of pyridoxol pyridoxal + NADPH
-
r

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyridoxine + NADP+ pyridoxine is identical with pyridoxol, equilibrium lies far to the side of pyridoxol Saccharomyces cerevisiae pyridoxal + NADPH
-
r
pyridoxine phosphate + NADP+
-
Saccharomyces cerevisiae pyridoxal 5'-phosphate + NADPH
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Saccharomyces cerevisiae

pH Range

pH Minimum pH Maximum Comment Organism
5 8 at pH 5.0 and 8.0 about 50% of activity maximum Saccharomyces cerevisiae