Information on EC 1.1.1.59 - 3-hydroxypropionate dehydrogenase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
1.1.1.59
-
RECOMMENDED NAME
GeneOntology No.
3-hydroxypropionate dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
3-hydroxypropanoate + NAD+ = 3-oxopropanoate + NADH + H+
show the reaction diagram
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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-
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redox reaction
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-
-
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reduction
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
acrylate degradation
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beta-alanine biosynthesis II
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beta-Alanine metabolism
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Metabolic pathways
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Propanoate metabolism
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propanoyl-CoA degradation II
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alanine metabolism
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SYSTEMATIC NAME
IUBMB Comments
3-hydroxypropanoate:NAD+ oxidoreductase
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CAS REGISTRY NUMBER
COMMENTARY hide
9028-59-5
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
; MmsB gene
-
-
Manually annotated by BRENDA team
MmsB gene
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
no activity in Desulfovibrio carbinolicus
-
-
-
Manually annotated by BRENDA team
no activity in Desulfovibrio fructosovorans
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-
-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(R)-3-hydroxyisobutyrate + NAD+
3-oxoisobutyrate + NADH + H+
show the reaction diagram
-
-
-
?
(S)-3-hydroxyisobutyrate + NAD+
3-oxoisobutyrate + NADH + H+
show the reaction diagram
-
-
-
?
3-hydroxyisobutyrate + NAD+
methylmalonate semialdehyde + NADH + H+
show the reaction diagram
-
-
-
-
r
3-hydroxypropanoate + NAD+
3-oxopropanoate + NADH + H+
show the reaction diagram
3-hydroxypropanoate + NAD+
malonate semialdehyde + NADH + H+
show the reaction diagram
-
-
-
-
r
3-hydroxypropanoate + NADP+
malonate semialdehyde + NADPH + H+
show the reaction diagram
-
-
-
-
r
L-serine + NAD+
3-oxo-L-alanine + NADH + H+
show the reaction diagram
-
-
-
?
additional information
?
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MmsB from Bacillus cereus exhibits 3-hydroxyisobutyrate dehydrogenase, EC 1.1.1.31, as well as 3-hydroxypropionate dehydrogenase activity
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3-hydroxypropanoate + NAD+
3-oxopropanoate + NADH + H+
show the reaction diagram
additional information
?
-
-
MmsB from Bacillus cereus exhibits 3-hydroxyisobutyrate dehydrogenase, EC 1.1.1.31, as well as 3-hydroxypropionate dehydrogenase activity
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-
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
no metal ion requirement
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ZnCl2
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0.2 mM, 60% inhibition
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.58
(R)-3-Hydroxyisobutyrate
at pH 9.5 and 22°C
0.13
(S)-3-hydroxyisobutyrate
at pH 9.5 and 22°C
5.37 - 20
3-hydroxypropanoate
16.8
3-hydroxypropionate
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pH 8.5, 37°C, KM-value for 3-hydroxyisobutyrate is approximately 20fold lower
46.7
L-serine
at pH 9.5 and 22°C
0.34 - 2.4
NAD+
0.25
NADP+
-
pH 8.5, 37°C
additional information
additional information
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steady-state kinetic analysis, overview
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.31
(R)-3-Hydroxyisobutyrate
Candida albicans
A0A1D8PQ07
at pH 9.5 and 22°C
2.48
(S)-3-hydroxyisobutyrate
Candida albicans
A0A1D8PQ07
at pH 9.5 and 22°C
0.21 - 30.97
3-hydroxypropanoate
0.21
3-hydroxypropionate
Bacillus cereus
-
pH 8.5, 37°C
9.41
L-serine
Candida albicans
A0A1D8PQ07
at pH 9.5 and 22°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.26
(R)-3-Hydroxyisobutyrate
Candida albicans
A0A1D8PQ07
at pH 9.5 and 22°C
44984
19.1
(S)-3-hydroxyisobutyrate
Candida albicans
A0A1D8PQ07
at pH 9.5 and 22°C
42816
0.0125 - 5.77
3-hydroxypropanoate
4480
0.0125
3-hydroxypropionate
Bacillus cereus
-
pH 8.5, 37°C
1632
0.202
L-serine
Candida albicans
A0A1D8PQ07
at pH 9.5 and 22°C
95
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.77
-
pH 8.5, 37°C
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.8 - 9
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 10
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; activity range
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35 - 45
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90% of maximal activity within this range
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
32000
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x * 32000, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45
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30 min, purified enzyme, completely stable; stable at 45°C for 30 min, about 40% activity after 3 min at 55°C
55
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3 min, purified enzyme, significant denaturation and inactivation
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
several weeks, frozen
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, hydrophobic interaction chromatography (Phenyl-Sepharose), anion exchange chromatography; native enzyme 100fold by ammonium sulfate fractionation, and hydrophobic interaction and anion exchange chromatography
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partially
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21 (DE3); MmsB gene, overexpression in Escherichia coli strain BL21, subcloning in strain DH5alpha
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