Information on EC 1.1.1.147 - 16alpha-hydroxysteroid dehydrogenase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.1.1.147
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RECOMMENDED NAME
GeneOntology No.
16alpha-hydroxysteroid dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
a 16alpha-hydroxysteroid + NAD(P)+ = a 16-oxosteroid + NAD(P)H + H+
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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redox reaction
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reduction
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SYSTEMATIC NAME
IUBMB Comments
16alpha-hydroxysteroid:NAD(P)+ 16-oxidoreductase
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CAS REGISTRY NUMBER
COMMENTARY hide
37250-74-1
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
16-ketotestosterone + NAD(P)H
16alpha-hydroxytestosterone + NAD(P)+
show the reaction diagram
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-
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r
17alpha-estradiol-16-one + NAD(P)H
16alpha-hydroxy-17alpha-estradiol + NAD(P)+
show the reaction diagram
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best substrate tested, changing configuration or oxidation state of the neighboring oxygen substituent at C-17 increases the rate of reduction
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r
17beta-estradiol-16-one + NAD(P)H
16alpha-hydroxy-17beta-estradiol + NAD(P)+
show the reaction diagram
3-hydroxy-estra-1,3,5(10)-triene-16,17-dione + NAD(P)H
3,16alpha-dihydroxy-estra-1,3,5(10)-triene-17-one + NAD(P)H
show the reaction diagram
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-
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r
3beta,17beta-dihydroxy-androst-5-en-16-one + NAD(P)H
3beta,16alpha,17beta-trihydroxyandrost-5-ene + NAD(P)+
show the reaction diagram
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low activity
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?
estra-1,3,5(10)-triene-3,16beta,17beta-triol + NAD(P)+
estra-1,3,5(10)-triene-3,17beta-diol-16-one + NAD(P)H
show the reaction diagram
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?
estra-1,3,5(10)triene-3,17-beta-diol-16-one + NAD(P)H
estra-1,3,5(10)triene-3,16alpha,17-triol + NAD(P)+
show the reaction diagram
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low activity
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r
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADH
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40% lower reaction rate than NADPH
NADPH
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40% greater reaction rate than NADH
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
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complete inhibition at 0.1 mM
2,2'-bipyridine
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19% inhibition at 0.1 mM
2-Iodoacetamide
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86% inhibition at 1 mM
8-hydroxy-5-quinolinesulfonate
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89% inhibition at 1 mM
Ag+
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78% inhibition at 0.01 mM
Cu2+
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98% inhibition at 0.01 mM
EDTA
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97% inhibition at 0.01 mM
N-ethylmaleimide
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94% inhibition at 0.1 mM
o-Iodosobenzoate
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complete inhibition at 0.1 mM
p-hydroxymercuribenzoate
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complete inhibition at 0.01 mM
Zn2+
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84% inhibition at 0.1 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.28
16-ketotestosterone
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0.07
17alpha-estradiol-16-one
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0.089
17beta-estradiol-16-one
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0.083
NADH
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0.041
NADPH
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.1 - 6.2
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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inducible by estrogen administration
Manually annotated by BRENDA team
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
extremely unstable, glycerol partially protects against inactivation
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial
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