General Stability | Organism |
---|---|
extremely unstable, glycerol partially protects against inactivation | Rattus sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | complete inhibition at 0.1 mM | Rattus sp. | |
2,2'-bipyridine | 19% inhibition at 0.1 mM | Rattus sp. | |
2-Iodoacetamide | 86% inhibition at 1 mM | Rattus sp. | |
8-hydroxy-5-quinolinesulfonate | 89% inhibition at 1 mM | Rattus sp. | |
Ag+ | 78% inhibition at 0.01 mM | Rattus sp. | |
Cu2+ | 98% inhibition at 0.01 mM | Rattus sp. | |
EDTA | 97% inhibition at 0.01 mM | Rattus sp. | |
N-ethylmaleimide | 94% inhibition at 0.1 mM | Rattus sp. | |
o-Iodosobenzoate | complete inhibition at 0.1 mM | Rattus sp. | |
p-hydroxymercuribenzoate | complete inhibition at 0.01 mM | Rattus sp. | |
Zn2+ | 84% inhibition at 0.1 mM | Rattus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.041 | - |
NADPH | - |
Rattus sp. | |
0.07 | - |
17alpha-estradiol-16-one | - |
Rattus sp. | |
0.083 | - |
NADH | - |
Rattus sp. | |
0.089 | - |
17beta-estradiol-16-one | - |
Rattus sp. | |
0.28 | - |
16-ketotestosterone | - |
Rattus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Rattus sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | inducible by estrogen administration | Rattus sp. | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
16-ketotestosterone + NAD(P)H | - |
Rattus sp. | 16alpha-hydroxytestosterone + NAD(P)+ | - |
r | |
16-oxo-17beta-estradiol + NADH + H+ | - |
Rattus sp. | 16alpha-hydroxy-17beta-estradiol + NAD+ | - |
r | |
17alpha-estradiol-16-one + NAD(P)H | best substrate tested, changing configuration or oxidation state of the neighboring oxygen substituent at C-17 increases the rate of reduction | Rattus sp. | 16alpha-hydroxy-17alpha-estradiol + NAD(P)+ | - |
r | |
17beta-estradiol-16-one + NAD(P)H | - |
Rattus sp. | 16alpha-hydroxy-17beta-estradiol + NAD(P)+ | - |
r | |
3-hydroxy-estra-1,3,5(10)-triene-16,17-dione + NAD(P)H + H+ | - |
Rattus sp. | 3,16alpha-dihydroxy-estra-1,3,5(10)-triene-17-one + NAD(P)+ | - |
r | |
3beta,17beta-dihydroxy-androst-5-en-16-one + NAD(P)H | low activity | Rattus sp. | 3beta,16alpha,17beta-trihydroxyandrost-5-ene + NAD(P)+ | - |
? | |
estra-1,3,5(10)-triene-3,16beta,17beta-triol + NAD(P)+ | - |
Rattus sp. | estra-1,3,5(10)-triene-3,17beta-diol-16-one + NAD(P)H | - |
? | |
estra-1,3,5(10)triene-3,17-beta-diol-16-one + NAD(P)H | low activity | Rattus sp. | estra-1,3,5(10)triene-3,16alpha,17-triol + NAD(P)+ | - |
r |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.1 | 6.2 | - |
Rattus sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | 40% lower reaction rate than NADPH | Rattus sp. | |
NADPH | 40% greater reaction rate than NADH | Rattus sp. |