2.7.2.10: phosphoglycerate kinase (GTP)

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For detailed information about phosphoglycerate kinase (GTP), go to the full flat file.

Reaction

Synonyms

3-phosphoglycerate kinase, kinase (phosphorylating), phosphoglycerate (guanosine triphosphate), kinase, phosphoglycerate (phosphorylating guanosine triphosphate), PGK

ECTree

     2 Transferases

         2.7 Transferring phosphorus-containing groups

             2.7.2 Phosphotransferases with a carboxy group as acceptor

                2.7.2.10 phosphoglycerate kinase (GTP)

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Results	in table
1	CAS Registry Number
1	Cloned(Commentary)
2	Cofactor
1	General Stability
21	KM Value [mM]
2	Localization
1	Metals/Ions
1	Natural Substrates/ Products (Substrates)
11	Organism
5	Protein Variants
2	Purification (Commentary)
1	Reaction
1	Reaction Type
3	Reference
1	Specific Activity [micromol/min/mg]
2	Storage Stability
9	Substrates and Products (Substrate)
2	Subunits
6	Synonyms
1	Systematic Name
1	Temperature Stability [°C]

Engineering

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Engineering on EC 2.7.2.10 - phosphoglycerate kinase (GTP)

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PROTEIN VARIANTS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

E309M

Entamoeba histolytica

-

increases catalytic efficiency (Vm/Km) with ADP/ATP as a result of both, increased Vm and decreased Km values

703640

E309Q

Entamoeba histolytica

-

increases catalytic efficiency (Vm/Km) with ADP/ATP as a result of both, increased Vm and decreased Km values

703640

V311L

Entamoeba histolytica

-

increases catalytic efficiency (Vm/Km) with ADP/ATP as a result of both, increased Vm and decreased Km values. Is monomeric as the wild-type

703640

Y239F

Entamoeba histolytica

-

increases catalytic efficiency (Vm/Km) with ADP/ATP as a result of both, increased Vm and decreased Km values

703640

Y239F/E309M

Entamoeba histolytica

-

higher catalytic efficiency in the double mutant is achieved mainly due to an increased affinity for ADP/ATP with a concomitant diminished affinity for GDP/GTP. Is monomeric as the wild-type

703640