2.7.1.43: glucuronokinase
This is an abbreviated version!
For detailed information about glucuronokinase, go to the full flat file.
Word Map on EC 2.7.1.43
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2.7.1.43
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glucuronic
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pyrophosphorylase
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pollen
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udp-sugar
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udp-glucuronic
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myo-inositol
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lilium
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sugar-1-phosphates
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longiflorum
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utp
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diagnostics
- 2.7.1.43
-
glucuronic
-
pyrophosphorylase
- pollen
- udp-sugar
-
udp-glucuronic
- myo-inositol
- lilium
-
sugar-1-phosphates
- longiflorum
- utp
- diagnostics
Reaction
Synonyms
At3g01640, AtGlcAK, AtGlcAK2, GKUP, GlcAK, GlcAK1, GlcAK2, glucuronokinase, glucuronokinase 1, glucuronokinase 2, glucuronokinase, glucurono-, glucuronokinase1, kinase, glucurono- (phosphorylating)
ECTree
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Engineering
Engineering on EC 2.7.1.43 - glucuronokinase
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additional information
generation of a frameshift mutations in the major expressed isoform glucuronokinase 1 to potentially redirect metabolites to ascorbic acid (AsA). But radiotracer experiments with 3H-myo-inositol reveal that the mutants in glucuronokinase 1 accumulate only glucuronic acid and incorporate less metabolite into cell wall polymers. AsA is not labelled, suggesting that Arabidopsis thaliana cannot efficiently use glucuronic acid for AsA biosynthesis. All four mutants in glucuronokinase as well as the wild type have the same level of AsA in leaves. Construction of CRISPR/Cas9 knockouts, mutants of GlcAK1 are generated using CRISPR/Cas9 technology including transformation by the Agrobacterium tumefaciens (strain GV3101) system, resulting in mutants glcak1-3 and glcak1-4. Analysis of T-DNA insertional mutants glcak1-1 and glcak1-2
additional information
identification of two T-DNA insertion homozygous mutants of AtGlcAK, atglcak-1 and atglcak-2. AtGlcAK mutants display hypersensitivity to abscisic acid (ABA) and reduced root development under water stress, rendering the plants more susceptible to drought stress. The atglcak mutants display shorter primary root and less lateral roots under osmotic stress. Loss-of-function of AtGlcAK Affects the expression of ABA-induced and sugar-metabolic genes. Phenotypes of atglcak-1 and atglcak-2, overview
additional information
identification of two T-DNA insertion homozygous mutants of AtGlcAK, atglcak-1 and atglcak-2. AtGlcAK mutants display hypersensitivity to abscisic acid (ABA) and reduced root development under water stress, rendering the plants more susceptible to drought stress. The atglcak mutants display shorter primary root and less lateral roots under osmotic stress. Loss-of-function of AtGlcAK Affects the expression of ABA-induced and sugar-metabolic genes. Phenotypes of atglcak-1 and atglcak-2, overview
additional information
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generation of a frameshift mutations in the major expressed isoform glucuronokinase 1 to potentially redirect metabolites to ascorbic acid (AsA). But radiotracer experiments with 3H-myo-inositol reveal that the mutants in glucuronokinase 1 accumulate only glucuronic acid and incorporate less metabolite into cell wall polymers. AsA is not labelled, suggesting that Arabidopsis thaliana cannot efficiently use glucuronic acid for AsA biosynthesis. All four mutants in glucuronokinase as well as the wild type have the same level of AsA in leaves. Construction of CRISPR/Cas9 knockouts, mutants of GlcAK1 are generated using CRISPR/Cas9 technology including transformation by the Agrobacterium tumefaciens (strain GV3101) system, resulting in mutants glcak1-3 and glcak1-4. Analysis of T-DNA insertional mutants glcak1-1 and glcak1-2
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additional information
-
identification of two T-DNA insertion homozygous mutants of AtGlcAK, atglcak-1 and atglcak-2. AtGlcAK mutants display hypersensitivity to abscisic acid (ABA) and reduced root development under water stress, rendering the plants more susceptible to drought stress. The atglcak mutants display shorter primary root and less lateral roots under osmotic stress. Loss-of-function of AtGlcAK Affects the expression of ABA-induced and sugar-metabolic genes. Phenotypes of atglcak-1 and atglcak-2, overview
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