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2.7.1.162: N-acetylhexosamine 1-kinase

This is an abbreviated version!
For detailed information about N-acetylhexosamine 1-kinase, go to the full flat file.

Word Map on EC 2.7.1.162

Reaction

ATP
+
N-acetyl-D-hexosamine
=
ADP
 +
N-acetyl-alpha-D-hexosamine 1-phosphate

Synonyms

BLLJ_1622, hexosamine kinase, lnpB, N-acetylhexosamine 1-kinase, N-acetylhexosamine 1-phosphate kinase, N-acetylhexosamine kinase, NahK, NahKATCC15697, NahK_15697

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.162 N-acetylhexosamine 1-kinase

Purification

Purification on EC 2.7.1.162 - N-acetylhexosamine 1-kinase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant C-terminally His6-tagged wild-type and SeMet-labeled NahK residues 1-359 enzymes from Escherichia coli by nickel affinity chromatography and gel filtration
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
The transformant is cultivated and protein expression is induced by the addition of isopropyl-1-thio-beta-D-galactoside. The cells are harvested by centrifugation and sonicated. Cell debris is removed by centrifugation and enzyme is purified on an Ni-nitrilotriacetic acid agarose gel.