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2.6.1.48: 5-aminovalerate transaminase

This is an abbreviated version!
For detailed information about 5-aminovalerate transaminase, go to the full flat file.

Word Map on EC 2.6.1.48

Reaction

5-Aminopentanoate
+
2-oxoglutarate
=
5-Oxopentanoate
 +
L-glutamate

Synonyms

5-aminovalerate aminotransferase, 5-aminovalerate transaminase, DavA, davT, dayT, delta-aminovalerate aminotransferase, delta-aminovalerate transaminase, delta-aminovaleric acid-glutamic acid transaminase, GabT, PA0266, putative 5-aminovalerate aminotransferase

ECTree

     2 Transferases
         2.6 Transferring nitrogenous groups
             2.6.1 Transaminases
                2.6.1.48 5-aminovalerate transaminase

Cloned

Cloned on EC 2.6.1.48 - 5-aminovalerate transaminase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloned by PCR and expressed in Escherichia coli as a C-terminal histidine-tagged fusion protein
expressed in Escherichia coli BL21(DE3) cells
gene davT identified using a transposon to generate transcriptional fusions by insertional mutagenesis
gene davT, recombinant expression of N-terminal His6-tagged enzyme in Corynebacterium glutamicum strain KCTC 1857, coexpression with glutarate semialdehyde dehydrogenase (davD, EC 1.2.1.20) from Pseudomonas putida, 4-aminobutyrate-2-oxoglutarate transaminase (gabT, EC 2.6.1.19) from Corynebacterium glutamicum, and NADP-dependent succinic semialdehyde dehydrogenase (gabD, EC 1.2.1.79) from Corynebacterium glutamicum, as well as N-terminal His6-tagged lysine 2-monooxygenase (davB, EC 1.13.12.2) from Pseudomonas putida
gene dayB, recombinant expression in Corynebacterium glutamicum, co-expression with Pseudomonas putida dayA and dayB genes encoding encoding lysine 2-monooxygenase and delta-aminovaleramidase, respectively, and Corynebacterium glutamicum gabD gene encoding glutarate semialdehyde dehydrogenase
gene dayT, recombinant expression in Corynebacterium glutamicum, co-expression with Pseudomonas putida dayA, dayB, and dayD genes encoding lysine 2-monooxygenase, delta-aminovaleramidase, and glutarate semialdehyde dehydrogenase, respectively