2.4.2.24: 1,4-beta-D-xylan synthase
This is an abbreviated version!
For detailed information about 1,4-beta-D-xylan synthase, go to the full flat file.
Word Map on EC 2.4.2.24
-
2.4.2.24
-
xylans
-
polysaccharide
-
glycosyltransferases
-
glucuronoxylan
-
arabinoxylans
-
glucuronyltransferase
-
hemicellulosic
-
rhamnogalacturonan
-
synthase-like
-
arabinan
-
mucilage
-
xylooligomers
-
biotechnology
- 2.4.2.24
- xylans
- polysaccharide
- glycosyltransferases
- glucuronoxylan
- arabinoxylans
-
glucuronyltransferase
-
hemicellulosic
- rhamnogalacturonan
-
synthase-like
- arabinan
- mucilage
-
xylooligomers
- biotechnology
Reaction
Synonyms
1,4-beta-xylan synthase, 1,4-beta-xylan xylosyltransferase, Arabidopsis thaliana cellulose synthase-like gene D family gene 5, ATCSLD, ATCSLD5, beta-(1,4)-xylosyltransferase, beta-1,4-xylan synthase, beta-1,4-XylT, EC 2.4.1.72, irregular xylem14, irregular xylem9, IRX10, IRX10L, IRX14, IRX9, PtrGT43B, PtrGT43C, synthase, 1,4-beta-xylan, xylan 1,4-beta-D-xylosyltransferase, xylan beta-1,4-xylosyltransferase, xylan synthase, xylan synthase-1, xylan synthetase, xylosyltransferase, uridine diphosphoxylose-1,4-beta-xylan, XylTase, Xys1
ECTree
2.4.2.24 1,4-beta-D-xylan synthaseAdvanced search results
results (
results (Substrates Products
Substrates Products on EC 2.4.2.24 - 1,4-beta-D-xylan synthase
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
top
SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
pyridylaminated beta-(1,4)-xylotriose + UDP-D-xylose
pyridylaminated beta-(1,4)-xylotetraose + UDP
-
-
-
-
?
UDP-D-galacturonic acid + (1,4-beta-D-galacturonic acid)n
UDP + (1,4-beta-D-galacturonic acid)n+1
homogalacturonan synthase activity (substrate: galacturonic acid, GalA) decreased in absence of ATCSLD5 (ATCSLD5-1 mutant line)
-
-
?
UDP-D-xylose + ((1,4-beta-D-xylooligosaccharide)n)-anthranilic acid
UDP + ((1,4-beta-D-xylooligosaccharide)n+1)-anthranilic acid
-
0.1 mM UDP-xylose + 0.5 mM anthranilic acid (AA) labelled xylooligosaccharides (Xyl(2) to Xyl(6)) as acceptor, 200 microgram microsomes, 21°C, pH 6.8, in presence of 5 mM MnCl2, 1 mM dithiothreitol, 0.5% Triton X-100
xylooligosaccharides (up to Xyl(12)) purified by HPLC and analysed by MALDI-TOF mass spectrometry
-
?
UDP-D-xylose + (1,4-beta-D-xylooligosaccharide)n
UDP + (1,4-beta-D-xylooligosaccharide)n+1
-
0.1 mM radiolabelled UDP-D-xylose + 0.2 microgram/microlitre (1,4-beta-D-xylopyranose)6, 100 microgram microsomes, 21°C, pH 6.8, in presence of 5 mM MnCl2, 1 mM dithiothreitol, 0.5% Triton X-100, reaction dependent on time and protein concentration
reduction in XylT activity in microsomal fraction of stems from irx9 mutant plants compared to wild-type plants in presence of exogenous substrates can be abolished by complementation with wild-type IRX9 gene, product quantification by scintillation counting
-
?
UDP-D-xylose + (1,4-beta-D-xylopyranose)6
UDP + (1,4-beta-D-xylopyranose)7
-
-
-
-
?
UDP-D-xylose + xylotetraose
?
microsomes isolated from transgenic BY2 cells are tested for XylT activity using xylotetraose (Xyl4) as an acceptor and UDP-xylose as a donor. XylT activity can not use the xylose monomer as an acceptor. Xylosyl residues are readily transferred onto xylooligomers ranging from 2 to 6 residues, resulting in the production of xylooligosaccharides with up to 11 xylosyl residues
-
-
?
UDP-D-xylose + (1,4-beta-D-xylan)n
UDP + (1,4-beta-D-xylan)n+1
-
-
-
?
UDP-D-xylose + (1,4-beta-D-xylan)n
UDP + (1,4-beta-D-xylan)n+1
-
catalyzes synthesis of the xylan main chain during the biogenesis of the plant cell wall
-
-
?
UDP-D-xylose + (1,4-beta-D-xylan)n
UDP + (1,4-beta-D-xylan)n+1
-
precipitation of product by 70% ethanol (final concentration) in presence of wheat arabinoxylan, followed by washing and scintillation counting, control for incorporation into (1,4-beta-D-xylan) by digestion with endo-xylanase
-
?
UDP-D-xylose + (1,4-beta-D-xylan)n
UDP + (1,4-beta-D-xylan)n+1
beta-(1,4)-xylan synthase activity, biosynthesis of xylan (major component of dicot wood)
-
-
?
UDP-D-xylose + (1,4-beta-D-xylan)n
UDP + (1,4-beta-D-xylan)n+1
microsomes isolated from transgenic BY2 cells are tested for XylT activity using xylotetraose (Xyl4) as an acceptor and UDP-xylose as a donor. Up to five xylosyl residues with beta-(1,4)-linkages are able to be incorporated by IRX9/IRX14-expressing microsomes
-
-
?
UDP-D-xylose + (1,4-beta-D-xylan)n
UDP + (1,4-beta-D-xylan)n+1
-
-
with high substrate concentration or prolonged incubation, three polysaccharides other than neutral xylan are synthesized, one of these is apparently a glycolipid, and the other two are apparently glucuronoxylans
?
UDP-D-xylose + (1,4-beta-D-xylan)n
UDP + (1,4-beta-D-xylan)n+1
-
substrate are e.g. higher xylooligosaccharide or xylans from different sources, chain elongation by formation of beta(1,4)-linkages, xylooligosaccharide substrates can be pyrimidylaminated or derivatized with 2-aminopyridine at their reducing ends and still serve as substrates, substrate can contain single 4-O-methyl-glucuronic acid residues
-
-
?
UDP-D-xylose + (1,4-beta-D-xylan)n
UDP + (1,4-beta-D-xylan)n+1
-
formation of hemicellulose of cell wall
-
-
?
UDP-D-xylose + (1,4-beta-D-xylan)n
UDP + (1,4-beta-D-xylan)n+1
-
-
-
-
?
UDP-D-xylose + (1,4-beta-D-xylan)n
UDP + (1,4-beta-D-xylan)n+1
-
catalyzes synthesis of the xylan main chain during the biogenesis of the plant cell wall
-
-
?
UDP-D-xylose + beta-1,4-xylotriose
UDP + ?
-
the reaction leads to extension of the beta-1,4-xylotriose by up to five xylose residues, beta-1,4-xylotetraose, beta-1,4-xylopentose, and beta-1,4-xylohexose
-
-
?
UDP-D-xylose + beta-1,4-xylotriose
UDP + ?
-
the reaction leads to extension of the beta-1,4-xylotriose by up to five xylose residues, beta-1,4-xylotetraose, beta-1,4-xylopentose, and beta-1,4-xylohexose
-
-
?
UDP-D-xylose + beta-1,4-xylotriose
UDP + ?
-
the reaction leads to extension of the beta-1,4-xylotriose by up to five xylose residues, beta-1,4-xylotetraose, beta-1,4-xylopentose, and beta-1,4-xylohexose
-
-
?
UDP-D-xylose + [(1->4)-beta-D-xylan]n
UDP + [(1->4)-beta-D-xylan]n+1
-
-
-
-
?
UDP-D-xylose + [(1->4)-beta-D-xylan]n
UDP + [(1->4)-beta-D-xylan]n+1
-
-
-
-
?
UDP-D-xylose + [(1->4)-beta-D-xylan]n
UDP + [(1->4)-beta-D-xylan]n+1
-
-
-
-
?
UDP-D-xylose + [(1->4)-beta-D-xylan]n
UDP + [(1->4)-beta-D-xylan]n+1
-
-
-
-
?
additional information
?
-
-
xylosyltransferase (XylT) activity, biosynthesis of xylan, a major component of dicot wood
-
-
?
additional information
?
-
-
synthesis of xylooligosaccharides from Xyl(6)-anthranilic acid by XylT activity in microsomal fractions of stems from irx9 mutant plants: after up to 5 h mixture of Xyl(7) to Xyl(9) only, Xyl(7) predominates
-
-
?
additional information
?
-
-
synthesis of xylooligosaccharides from Xyl(6)-anthranilic acid by XylT activity in microsomal fractions of stems from wild-type plants: after 2 h Xyl(7) to Xyl(9) predominate, after 5 h increased proportion of Xyl(10) to Xyl(12)
-
-
?
additional information
?
-
-
Xyl(1)-anthranilic acid is not acceptor for XylT activity in microsomal fractions of stems from wild-type plants
-
-
?
additional information
?
-
-
Xyl(2)-anthranilic acid is a weak acceptor for XylT activity in microsomal fractions of stems from wild-type plants
-
-
?
additional information
?
-
-
Xyl(3)-anthranilic acid to Xyl(6)-anthranilic acid are readily accepted by XylT activity in microsomal fractions of stems from wild-type plants
-
-
?
additional information
?
-
-
Xyl(4)-anthranilic acid is acceptor for synthesis of xylooligosaccharides of sizes from Xyl(5) to Xyl(11) by XylT activity in microsomal fractions of stems from wild-type plants within 2 h, prolonged incubation time (5-8 h) leads to increased proportion of Xyl(9) to Xyl(11) but not to synthesis of longer xylooligosaccharides
-
-
?
additional information
?
-
-
no activity with UDP-glucose, UDP-arabinopyranose, or UDP-arabinofuranose
-
-
?
additional information
?
-
-
metabolic flux control and alterations during light reduction
-
-
?
additional information
?
-
-
no activity with UDP-glucose, UDP-arabinopyranose, or UDP-arabinofuranose
-
-
?
additional information
?
-
-
no activity with UDP-glucose, UDP-arabinopyranose, or UDP-arabinofuranose
-
-
?
