2.4.1.67: galactinol-raffinose galactosyltransferase

This is an abbreviated version!
For detailed information about galactinol-raffinose galactosyltransferase, go to the full flat file.

Reaction

Synonyms

AmSTS1, galactinol:raffinose-6-galactosyl-transferase, galactosyltransferase, galactinol-raffinose, More, RFO synthase/galactosylhydrolase, RS4, stachyose synthase, stachyose synthetase, StaS, STS

ECTree

     2 Transferases

         2.4 Glycosyltransferases

             2.4.1 Hexosyltransferases

                2.4.1.67 galactinol-raffinose galactosyltransferase

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Results	in table
7	Activating Compound
26	AA Sequence
1	CAS Registry Number
6	Cloned(Commentary)
6	Expression
10	General Information
2	General Stability
22	Inhibitors
3	Ki Value [mM]
19	KM Value [mM]
4	Localization
5	Metals/Ions
11	Molecular Weight [Da]
15	Natural Substrates/ Products (Substrates)
46	Organism
3	Pathway
14	pH Optimum
3	pH Range
1	pH Stability
3	Protein Variants
9	Purification (Commentary)
2	Reaction
1	Reaction Type
20	Reference
41	Source Tissue
38	Specific Activity [micromol/min/mg]
3	Storage Stability
67	Substrates and Products (Substrate)
8	Subunits
32	Synonyms
1	Systematic Name
12	Temperature Optimum [°C]
4	Temperature Range [°C]

Substrates Products

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of the Enzyme Summary Page.

Substrates Products on EC 2.4.1.67 - galactinol-raffinose galactosyltransferase

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SUBSTRATE

PRODUCT                       

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

1-alpha-D-galactosyl-myo-inositol + D-ononitol

myo-inositol + alpha-D-galactosyl-D-ononitol

7 entries

1-alpha-D-galactosyl-myo-inositol + D-pinitol

myo-inositol + galactopinitol A

3 entries

1-alpha-D-galactosyl-myo-inositol + galactopinitol A

myo-inositol + ciceritol

Lens culinaris

-

-

638266

i.e. O-alpha-D-galactopyranosyl-(1,6)-O-alpha-D-galactopyranosyl-(1,2)-4-O-methyl-D-chiro-inositol

638266

?

1-alpha-D-galactosyl-myo-inositol + H2O

myo-inositol + D-galactose

2 entries

1-alpha-D-galactosyl-myo-inositol + melibiose

myo-inositol + alpha-D-galactosyl-6-O-D-galactosyl-alpha-D-glucose

2 entries

1-alpha-D-galactosyl-myo-inositol + myo-inositol

myo-inositol + 1-alpha-D-galactosyl-myo-inositol

3 entries

1-alpha-D-galactosyl-myo-inositol + raffinose

myo-inositol + stachyose

16 entries

1-alpha-D-galactosyl-myo-inositol + sequoyitol

myo-inositol + alpha-D-galactosyl-sequoyitol

5 entries

1-alpha-D-galactosyl-myo-inositol + stachyose

myo-inositol + verbascose

Pisum sativum

Q93XK2

12.1% activity compared to raffinose

638268

-

638268

?

1-alpha-D-galactosyl-myo-inositol + verbascose

myo-inositol + ajugose

Pisum sativum

Q93XK2

-

638268

-

638268

?

alpha-D-galactosyl-(1->3)-1D-myo-inositol + raffinose

myo-inositol + stachyose

4 entries

alpha-D-galactosyl-D-ononitol + raffinose

D-ononitol + stachyose

3 entries

alpha-D-galactosyl-D-ononitol + stachyose

D-ononitol + verbascose

Pisum sativum

Q93XK2

9.9% activity compared to 1-alpha-D-galactosyl-myo-inositol

638268

-

638268

?

galactinol + raffinose

?

Cucumis sativus

-

assay at pH 7.0, 25°C, 90 min, reaction terminated by addition of NaOH

706231

-

-

?

galactinol + raffinose

myo-inositol + stachyose

Cucumis sativus

-

assay at pH 7.0, 25°C, 90 min, reaction terminated by addition of NaOH

706750

-

-

?

p-nitrophenyl-alpha-D-galactopyranoside + raffinose

p-nitrophenol + stachyose

Cucurbita pepo

-

about 10% activity compared to 1-alpha-D-galactosyl-myo-inositol

638261

-

638261

r

stachyose + stachyose

raffinose + verbascose

Pisum sativum

Q93XK2

-

638268

-

638268

?

additional information

?

-

15 entries

1-alpha-D-galactosyl-myo-inositol + D-ononitol

myo-inositol + alpha-D-galactosyl-D-ononitol

Lens culinaris

-

ononitol is 1-D-4-O-methyl-myo-inositol

638266

-

638266

r

1-alpha-D-galactosyl-myo-inositol + D-ononitol

myo-inositol + alpha-D-galactosyl-D-ononitol

Pisum sativum

Q93XK2

144.6% activity compared to raffinose

638268

-

638268

?

1-alpha-D-galactosyl-myo-inositol + D-ononitol

myo-inositol + alpha-D-galactosyl-D-ononitol

Pisum sativum

Q93XK2

ononitol is 1-D-4-O-methyl-myo-inositol

638268

-

638268

?

1-alpha-D-galactosyl-myo-inositol + D-ononitol

myo-inositol + alpha-D-galactosyl-D-ononitol

Vigna angularis

Q9SBZ0

recombinant enzyme

638267

-

638267

?

1-alpha-D-galactosyl-myo-inositol + D-ononitol

myo-inositol + alpha-D-galactosyl-D-ononitol

Vigna angularis

-

ononitol is 1-D-4-O-methyl-myo-inositol

638265

-

638265

r

1-alpha-D-galactosyl-myo-inositol + D-ononitol

myo-inositol + alpha-D-galactosyl-D-ononitol

Vigna angularis

Q9SBZ0

ononitol is 1-D-4-O-methyl-myo-inositol

638267

-

638267

?

1-alpha-D-galactosyl-myo-inositol + D-ononitol

myo-inositol + alpha-D-galactosyl-D-ononitol

Vigna angularis

-

biosynthesis of galactosylononitol

638265

-

-

?

1-alpha-D-galactosyl-myo-inositol + D-pinitol

myo-inositol + galactopinitol A

Lens culinaris

-

D-pinitol is 1-D-3-O-methyl-chiro-inositol

638266

i.e. O-alpha-D-galactopyranosyl-(1,2)-4-O-methyl-D-chiro-inositol

638266

r

1-alpha-D-galactosyl-myo-inositol + D-pinitol

myo-inositol + galactopinitol A

Pisum sativum

Q93XK2

11.2% activity compared to raffinose

638268

i.e. O-alpha-D-galactopyranosyl-(1,2)-4-O-methyl-D-chiro-inositol

638268

?

1-alpha-D-galactosyl-myo-inositol + D-pinitol

myo-inositol + galactopinitol A

Pisum sativum

Q93XK2

D-pinitol is 1-D-3-O-methyl-chiro-inositol

638268

i.e. O-alpha-D-galactopyranosyl-(1,2)-4-O-methyl-D-chiro-inositol

638268

?

1-alpha-D-galactosyl-myo-inositol + H2O

myo-inositol + D-galactose

Cucurbita pepo

-

hydrolysis

638261

-

638261

?

1-alpha-D-galactosyl-myo-inositol + H2O

myo-inositol + D-galactose

Pisum sativum

Q93XK2

hydrolysis

638268

-

638268

?

1-alpha-D-galactosyl-myo-inositol + melibiose

myo-inositol + alpha-D-galactosyl-6-O-D-galactosyl-alpha-D-glucose

Cucurbita pepo

-

10% activity compared to raffinose

638261

i.e. manninotriose

638261

ir

1-alpha-D-galactosyl-myo-inositol + melibiose

myo-inositol + alpha-D-galactosyl-6-O-D-galactosyl-alpha-D-glucose

Phaseolus vulgaris

-

25% activity compared to raffinose

638260

-

-

?

1-alpha-D-galactosyl-myo-inositol + myo-inositol

myo-inositol + 1-alpha-D-galactosyl-myo-inositol

Vigna angularis

-

exchange reaction

638265

-

638265

r

1-alpha-D-galactosyl-myo-inositol + myo-inositol

myo-inositol + 1-alpha-D-galactosyl-myo-inositol

Vigna angularis

-

several isomeric inositols and inositol O-methyl-ethers are substrates, some are inactive, overview

638265

-

638265

r

1-alpha-D-galactosyl-myo-inositol + myo-inositol

myo-inositol + 1-alpha-D-galactosyl-myo-inositol

Vigna angularis

-

2.25fold increased activity compared to raffinose

638265

-

638265

r

1-alpha-D-galactosyl-myo-inositol + raffinose

myo-inositol + stachyose

Ajuga reptans

-

i.e. galactinol

638263

-

638263

?

1-alpha-D-galactosyl-myo-inositol + raffinose

myo-inositol + stachyose

Ajuga reptans

-

i.e. galactinol

638264

-

638264

?

1-alpha-D-galactosyl-myo-inositol + raffinose

myo-inositol + stachyose

Ajuga reptans

-

seasonal variations in metabolism of raffinose family oligosaccharides, i.e. RFO

638263

-

-

?

1-alpha-D-galactosyl-myo-inositol + raffinose

myo-inositol + stachyose

Ajuga reptans

-

intracellular compartmentation of raffinose family oligosaccharide metabolism

638264

-

-

?

1-alpha-D-galactosyl-myo-inositol + raffinose

myo-inositol + stachyose

Cucumis melo

-

i.e. galactinol

638262

-

638262

?

1-alpha-D-galactosyl-myo-inositol + raffinose

myo-inositol + stachyose

Cucumis melo

-

involved in biosynthesis of sucrosyl-oligosaccharides

638262

-

-

?

1-alpha-D-galactosyl-myo-inositol + raffinose

myo-inositol + stachyose

Cucurbita pepo

-

i.e. galactinol

638261

-

638261

r

1-alpha-D-galactosyl-myo-inositol + raffinose

myo-inositol + stachyose

Lens culinaris

-

-

638266

-

-

?

1-alpha-D-galactosyl-myo-inositol + raffinose

myo-inositol + stachyose

Lens culinaris

-

i.e. galactinol

638266

-

638266

?

1-alpha-D-galactosyl-myo-inositol + raffinose

myo-inositol + stachyose

Phaseolus vulgaris

-

-

638260

-

-

?

1-alpha-D-galactosyl-myo-inositol + raffinose

myo-inositol + stachyose

Phaseolus vulgaris

-

i.e. galactinol

638260

-

638260

?

1-alpha-D-galactosyl-myo-inositol + raffinose

myo-inositol + stachyose

Pisum sativum

Q93XK2

i.e. galactinol

638268

-

638268

?

1-alpha-D-galactosyl-myo-inositol + raffinose

myo-inositol + stachyose

Vigna angularis

-

-

638265, 723481

-

-

?

1-alpha-D-galactosyl-myo-inositol + raffinose

myo-inositol + stachyose

Vigna angularis

Q9SBZ0

native and recombinant enzyme

638267

-

638267

?

1-alpha-D-galactosyl-myo-inositol + raffinose

myo-inositol + stachyose

Vigna angularis

-

i.e. galactinol

638265

-

638265

?

1-alpha-D-galactosyl-myo-inositol + raffinose

myo-inositol + stachyose

Vigna angularis

Q9SBZ0

i.e. galactinol

638267

-

638267

?

1-alpha-D-galactosyl-myo-inositol + sequoyitol

myo-inositol + alpha-D-galactosyl-sequoyitol

Lens culinaris

-

sequoyitol is 5-O-methyl-myo-inositol

638266

-

638266

?

1-alpha-D-galactosyl-myo-inositol + sequoyitol

myo-inositol + alpha-D-galactosyl-sequoyitol

Pisum sativum

Q93XK2

sequoyitol is 5-O-methyl-myo-inositol

638268

-

638268

?

1-alpha-D-galactosyl-myo-inositol + sequoyitol

myo-inositol + alpha-D-galactosyl-sequoyitol

Pisum sativum

Q93XK2

156.6% activity compared to raffinose

638268

-

638268

?

1-alpha-D-galactosyl-myo-inositol + sequoyitol

myo-inositol + alpha-D-galactosyl-sequoyitol

Vigna angularis

-

sequoyitol is 5-O-methyl-myo-inositol

638265

-

638265

?

1-alpha-D-galactosyl-myo-inositol + sequoyitol

myo-inositol + alpha-D-galactosyl-sequoyitol

Vigna angularis

-

1.3fold increased activity compared to raffinose

638265

-

638265

?

alpha-D-galactosyl-(1->3)-1D-myo-inositol + raffinose

myo-inositol + stachyose

Arabidopsis thaliana

-

-

736162

-

-

?

alpha-D-galactosyl-(1->3)-1D-myo-inositol + raffinose

myo-inositol + stachyose

Arabidopsis thaliana Col-0

-

-

736162

-

-

?

alpha-D-galactosyl-(1->3)-1D-myo-inositol + raffinose

myo-inositol + stachyose

Craterostigma plantagineum

-

-

736151

-

-

?

alpha-D-galactosyl-(1->3)-1D-myo-inositol + raffinose

myo-inositol + stachyose

Glycine max

-

-

737276

-

-

?

alpha-D-galactosyl-D-ononitol + raffinose

D-ononitol + stachyose

Pisum sativum

Q93XK2

65.8% activity compared to 1-alpha-D-galactosyl-myo-inositol

638268

-

638268

?

alpha-D-galactosyl-D-ononitol + raffinose

D-ononitol + stachyose

Vigna angularis

-

-

638265

-

638265

?

alpha-D-galactosyl-D-ononitol + raffinose

D-ononitol + stachyose

Vigna angularis

Q9SBZ0

recombinant enzyme

638267

-

638267

?

additional information

?

-

Ajuga reptans

-

stachyose is synthesized in different tissues, depending on its physiological fate, storage stachyose is synthesized in the mesophyll cell cytosol, from which it is immediately uptaken into the vacuole for elongation and storage, transport stachyose is synthesized in the intermediary cells

638264

-

-

?

additional information

?

-

Arabidopsis thaliana

-

the multifunctional enzyme is a raffinose and high affinity stachyose synthase, EC 2.4.1.82 and EC 2.4.1.67, as well as stachyose and galactinol specific galactosylhydrolase

736162

-

-

?

additional information

?

-

Arabidopsis thaliana Col-0

-

the multifunctional enzyme is a raffinose and high affinity stachyose synthase, EC 2.4.1.82 and EC 2.4.1.67, as well as stachyose and galactinol specific galactosylhydrolase

736162

-

-

?

additional information

?

-

Cucurbita pepo

-

high donor specificity: UDP-galactose or melibiose are no galactosyl-donors

638261

-

-

?

additional information

?

-

Cucurbita pepo

-

no substrates are sucrose, maltose, fructose, cellobiose, gentiobiose, melizitose, trehalose

638261

-

-

?

additional information

?

-

Cucurbita pepo

-

no substrates: lactose, glucose, maltotriose, manninotriose

638261

-

-

?

additional information

?

-

Lens culinaris

-

substrate specificity, overview

638266

-

-

?

additional information

?

-

Phaseolus vulgaris

-

no substrates are sucrose, maltose, fructose, cellobiose, gentiobiose, melizitose, trehalose

638260

-

-

?

additional information

?

-

Phaseolus vulgaris

-

poor substrates are glucose, galactose, lactose

638260

-

-

?

additional information

?

-

Phaseolus vulgaris

-

glycerol is no substrate

638260

-

-

?

additional information

?

-

Pisum sativum

Q93XK2

native and recombinant enzyme show similar activity

638268

-

-

?

additional information

?

-

Pisum sativum

-

native and recombinant enzyme show similar activity

638268

-

-

?

additional information

?

-

Pisum sativum

Q93XK2

sucrose is no acceptor substrate

638268

-

-

?

additional information

?

-

Pisum sativum

-

sucrose is no acceptor substrate

638268

-

-

?

additional information

?

-

Vigna angularis

-

substrate specificity, overview

638265

-

-

?