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2.4.1.64: alpha,alpha-trehalose phosphorylase

This is an abbreviated version!
For detailed information about alpha,alpha-trehalose phosphorylase, go to the full flat file.

Word Map on EC 2.4.1.64

Reaction

alpha,alpha-trehalose
+
phosphate
=
D-glucose
+
beta-D-glucose 1-phosphate

Synonyms

Bsel_1207, inverting trehalose phosphorylase, phosphorylase, trehalose, TbTP, trehalose phosphorylase

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.64 alpha,alpha-trehalose phosphorylase

General Stability

General Stability on EC 2.4.1.64 - alpha,alpha-trehalose phosphorylase

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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
enzyme retains full activity during 5 months at -14°C with several freezings and thawings, in presence of 2 mM phosphate buffer, pH 7.0, beta-D-glucose-1-phosphate and alpha-D-glucose-1-phosphate
-
loss of activity when extracts are desalted by dialysis or passage through Sephadex G-25 in presence of water. When dialysis is carried out against 5 mM NaCl, glucose 6-phosphate, EDTA, or imidazole-HCl buffer, pH 7.0, the enzymatic activity is maintained only for a few h
-