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2.4.1.257: GDP-Man:Man2GlcNAc2-PP-dolichol alpha-1,6-mannosyltransferase

This is an abbreviated version!
For detailed information about GDP-Man:Man2GlcNAc2-PP-dolichol alpha-1,6-mannosyltransferase, go to the full flat file.

Reaction

GDP-alpha-D-mannose
+
alpha-D-Man-(1->3)-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol
=
GDP
 +
alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol

Synonyms

Alg2, Alg2 mannosyltransferase, Alg2 MTase, More, MTase, scAlg2

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.257 GDP-Man:Man2GlcNAc2-PP-dolichol alpha-1,6-mannosyltransferase

Cloned

Cloned on EC 2.4.1.257 - GDP-Man:Man2GlcNAc2-PP-dolichol alpha-1,6-mannosyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene ALG2, cloning from Saccharomyces cerevisiae strain W303a, recombinant expression of wild-type and mutant N-terminal thioredoxin (Trx)-His6-tagged enzymes in Escherichia coli strain DE3
gene ALG2, transient recombinant expression of N-terminally His10-YFP tagged enzyme in HEK-293 cells
overexpression in Escherichia coli. Two Alg2 constructs are expressed and isolated, one with the N-terminal TRX domain and C-terminal His and V5 epitope tags and the other with only an N-terminal His tag