2.3.1.175: deacetylcephalosporin-C acetyltransferase

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For detailed information about deacetylcephalosporin-C acetyltransferase, go to the full flat file.

Reaction

Synonyms

acetyl coenzyme A: deacetylacephalosporin C O-acetyltransferase, acetyl coenzyme A: deacetylcephalosporin C O-acetyltransferase, acetyl coenzyme A:DAC acetyltransferase, acetyl-CoA:DAC acetyltransferase, acetyl-CoA:DAC O-acetyltransferase, acetyl-CoA:DAC-acetyltransferase, acetyl-CoA:deacetylcephalosporin C O-acetyltransferase, acetyl-CoA:deacetylcephalosporin-C acetyltransferase, cefG, CPC acetylhydrolase, DAC acetyltransferase, DAC-AT, DCPC-ATF, deacetylcephalosporin C acetyltransferase, deacetylcephalosporin-C acetyltransferase

ECTree

     2 Transferases

         2.3 Acyltransferases

             2.3.1 Transferring groups other than aminoacyl groups

                2.3.1.175 deacetylcephalosporin-C acetyltransferase

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Results	in table
4	AA Sequence
3	Application
1	CAS Registry Number
8	Cloned(Commentary)
1	Crystallization (Commentary)
5	Inhibitors
4	KM Value [mM]
1	Localization
4	Molecular Weight [Da]
4	Natural Substrates/ Products (Substrates)
12	Organism
4	Pathway
36	PDB ID
3	pH Optimum
1	pH Range
2	pI Value
1	Posttranslational Modification
3	Purification (Commentary)
1	Reaction
11	Reference
1	Source Tissue
6	Specific Activity [micromol/min/mg]
9	Substrates and Products (Substrate)
2	Subunits
22	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
1	Temperature Range [°C]

Cloned

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Cloned on EC 2.3.1.175 - deacetylcephalosporin-C acetyltransferase

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

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Acremonium chrysogenum

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657712, 658486

constructions starting at each of the three in-frame ATG codons of the Acremonium chrysogenum cefG gene (Met1, Met46 and Met60) are expressed in Escherichia coli, obtaining proteins of 49000 Da, 44000 Da and 43000 Da, respectively. All three proteins show deacetylcephalosporin C acetyltransferase activity, separately or in combination

Acremonium chrysogenum

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657802

expression in Penicillium chrysogenum

Acremonium chrysogenum

-

659005

expression in yeast, the leader peptide is not essential for expression of the enzyme activity

Acremonium chrysogenum

-

657711

expression of the cefG gene from the promoters of 1. the glyceraldehyde-3-phosphate dehydrogenase gene of Aspergillus nidulans, 2. the glucoamylase gene of Aspergillus niger, 3. the glutamate dehydrogenase and 4. the isopenicillin N synthase genes of Penicillium chrysogenum, leads to very high steady-state levels of cefG transcript and to increased deacetylcephalosporin-C acetyltransferase protein concentration

Acremonium chrysogenum

-

657617

into the pTWIN vector for expression in Escherichia coli BL21DE3 cells

Acremonium chrysogenum

P39058

688409

Penicillium chrysogenum strains expressing cefD1, cefD2, cefEF, and cefG genes cloned form Acremonium chrysogenum are constructed, deacetylcephalosporin-C acetyltransferase is coded by the cefG gene

Acremonium chrysogenum

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673028