2.1.1.221: tRNA (guanine9-N1)-methyltransferase

This is an abbreviated version!
For detailed information about tRNA (guanine9-N1)-methyltransferase, go to the full flat file.

Word Map on EC 2.1.1.221

2.1.1.221

microcephaly

archaea

disability

intellectual

purine

stature

eukarya

n1-methylation

monogenic

puberty

Reaction

Synonyms

EC 2.1.1.31, hTRMT10A, m1G9 methyltransferase, m1G9 MTase, m1R9-specific TkTrm10, More, ScTrm10, SPOUT MTase, TK0422p, TkTrm10, Trm10, Trm10p (ambiguous), TRMT10A, Trmt10C, tRNA (guanine-N(1)-)-methyltransferase, tRNA m1G9 methyltransferase, tRNA m1G9 MTase, tRNA m1G9 SPOUT methyltransferase, tRNA m1R9 methyltransferase, tRNA(m1G9/m1A9)-methyltransferase, tRNA(m1G9/m1A9)MTase

ECTree

2 Transferases

2.1 Transferring one-carbon groups

2.1.1 Methyltransferases

2.1.1.221 tRNA (guanine9-N1)-methyltransferase

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Results	in table
4101	AA Sequence
11	Cloned(Commentary)
14	Cofactor
3	Crystallization (Commentary)
78	Disease/ Diagnostics
48	General Information
6	kcat/KM [mM/s]
14	KM Value [mM]
2	Localization
8	Metals/Ions
39	Natural Substrates/ Products (Substrates)
29	Organism
16	PDB ID
8	pH Optimum
2	pH Range
44	Protein Variants
10	Purification (Commentary)
4	Reaction
23	Reference
8	Source Tissue
70	Substrates and Products (Substrate)
5	Subunits
65	Synonyms
1	Systematic Name
8	Temperature Optimum [°C]
9	Turnover Number [1/s]

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Cloned on EC 2.1.1.221 - tRNA (guanine9-N1)-methyltransferase

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expression in Escherichia coli

2 entries

gene TK0422, sequence comparisons, recombinant expression of N-terminally His6-tagged protein in Escherichia coli

Thermococcus kodakarensis

Q5JD38

756865

gene Trm10, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli

Saccharomyces cerevisiae

Q12400

757847

gene trm10, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli

2 entries

gene TRMT10A or RG9MTD2, genotyping

Homo sapiens

Q8TBZ6

737064

gene TrmT10A, recombinant expression of His-tagged enzyme in Escherichia coli

Homo sapiens

Q8TBZ6

757847

overexpression in Escherichia coli

Saccharomyces cerevisiae

Q12400

663384

recombinant expression of N-terminally His-tagged enzyme Trm10 in Escherichia coli strain BL21/Gold(DE3)

Saccharomyces cerevisiae

Q12400

736903

recombinant expression of N-terminally His-tagged enzyme Trm10 in Escherichia coli strain BL21/Gold(DE3), selenomethionine-labeled spTrm10_74 (residues 74-281) is expressed in Escherichia coli strain B834, recombinant wild-type and mutant Q226A TrmT10C (residues 40-403) is coexpressed with His-tagged protein SDR5C1

Schizosaccharomyces pombe

O14214

736903