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2.1.1.22: carnosine N-methyltransferase

This is an abbreviated version!
For detailed information about carnosine N-methyltransferase, go to the full flat file.

Word Map on EC 2.1.1.22

Reaction

S-adenosyl-L-methionine
+
carnosine
=
S-adenosyl-L-homocysteine
+
anserine

Synonyms

C9orf41, Carmt1, carnosine N-methyltransferase 2, UPF0586 protein C9orf41 homolog, YNL092W

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.22 carnosine N-methyltransferase

Engineering

Engineering on EC 2.1.1.22 - carnosine N-methyltransferase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C123A
mutation in disulfide bridge, displays strongly enhanced activity compared to that of wild-type
C238A
mutation in disulfide bridge, significantly reduces the activity
D278A/T279A
mutant barely displays any affinity to SAM
E167A
mutant barely displays any affinity to SAM
H309A
about 40% of wild-type activity
L307F
activity similar to wild-type
N274A
substantial reduction in expression level. Mutant barely displays any affinity to SAM
Q108A
mutant barely displays any affinity to SAM
R111A
mutant barely displays any affinity to SAM
S104A
activity similar to wild-type
S234A
mutant barely displays any affinity to SAM
Y286A
about 10fold lower affinity to SAM than wild-type
Y371A
substantial reduction in expression level, mutant barely displays any affinity to SAM
C123A
-
mutation in disulfide bridge, displays strongly enhanced activity compared to that of wild-type
-
E167A
-
mutant barely displays any affinity to SAM
-
H309A
-
about 40% of wild-type activity
-
R111A
-
mutant barely displays any affinity to SAM
-
S234A
-
mutant barely displays any affinity to SAM
-