2.1.1.193: 16S rRNA (uracil1498-N3)-methyltransferase
This is an abbreviated version!
For detailed information about 16S rRNA (uracil1498-N3)-methyltransferase, go to the full flat file.
Reaction
Synonyms
16S rRNA methyltransferase, DUF558 protein, m3U1498 specific methyltransferase, PA0419, RsmE, Rv2372c, YggJ
ECTree
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Crystallization
Crystallization on EC 2.1.1.193 - 16S rRNA (uracil1498-N3)-methyltransferase
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sitting drop vapor diffusion method at room temperature, 0.15 M potassium thiocyanate and 24% w/v PEG monomethyl ether 2000, 3-4 days, crystal soaking in S-adenosyl-L-methionine solution or cocrystallization of enzyme and cofactor are not successful, X-ray diffraction structure determination and analysis at 2.25 A resolution, molecular replacement
sitting drop vapor diffusion method, using 100 mM Tris-HCl pH 8.0, 1.5 M potassium acetate, at 24°C