1.5.3.14: polyamine oxidase (propane-1,3-diamine-forming)
This is an abbreviated version!
For detailed information about polyamine oxidase (propane-1,3-diamine-forming), go to the full flat file.
Word Map on EC 1.5.3.14
-
1.5.3.14
-
spermine
-
oxidases
-
back-conversion
-
apoplastic
-
thermospermine
-
putrescine
-
tabacum
-
fad-dependent
-
tunnel
-
barley
-
mesocotyl
-
1,3-diaminopropane
-
tetraamines
-
aminoaldehyde
-
1,8-diaminooctane
-
h2o2-producing
-
u-shaped
-
guazatine
- 1.5.3.14
- spermine
- oxidases
-
back-conversion
- apoplastic
- thermospermine
- putrescine
- tabacum
-
fad-dependent
-
tunnel
- barley
- mesocotyl
- 1,3-diaminopropane
-
tetraamines
- aminoaldehyde
- 1,8-diaminooctane
-
h2o2-producing
-
u-shaped
- guazatine
Reaction
Synonyms
EC 1.5.3.11, flavin-containing polyamine oxidase, maize PAO, maize polyamine oxidase, MPAO, OsPAO7, PAO, ZmPAO
ECTree
Advanced search results
Crystallization
Crystallization on EC 1.5.3.14 - polyamine oxidase (propane-1,3-diamine-forming)
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
crystallised by the hanging drop vapour-diffusion method, with the protein solution consisting of 5 mg enzyme/ml in 300 mM NaCl and 50 mM sodium phosphate buffer, pH 6.0. Crystal structure of polyamine oxidase is determined to a resolution of 1.9 A. The enzyme contains two domains, which define a remarkable 30 A long U-shaped catalytic tunnel at their interface. The structure of PAO in complex with the inhibitor MDL72527 reveals the residues forming the catalytic machinery and unusual enzyme-inhibitor CH...OH bonds. A ring of glutamate and aspartate residues surrounding one of the two tunnel openings contributes to the steering of the substrate towards the inside of the tunnel
purified recombinant mutant ZmPAO-K300M, hanging drop vapor diffusion method, mixing of 9.0 mg/ml protein in 50 mM sodium acetate, pH 5.5, with an equal volume of reservoir solution containing ammonium sulfate in a concentration range 2.22.8 m and 100 mm sodium acetate, pH 4.6, 20°C, two weeks, X-ray diffraction structure determination and analysis at 2.9 A resolution,mlecular displacement, modelling
-