1.2.5.3: aerobic carbon monoxide dehydrogenase
This is an abbreviated version!
For detailed information about aerobic carbon monoxide dehydrogenase, go to the full flat file.
Reaction
Synonyms
aerobic Mo/Cu-containing CO dehydrogenase, Carbon monoxide dehydrogenase, CO dehydrogenase, CODH, CoxS, coxSML, CutL, CutM, CutS, EC 1.2.2.4, EC 1.2.3.10, Mo-CODH, Mo-Cu carbon monoxide dehydrogenase, Mo/Cu CODH, MoCu-CODH, molybdenum- and copper-containing carbon monoxide dehydrogenase, molybdenum- and copper-dependent CO dehydrogenase, molybdenum-containing carbon monoxide dehydrogenase, molybdenum-containing CO dehydrogenase, molybdenum-copper carbon monoxide dehydrogenase, molybdenum-copper CO dehydrogenase, molybdenum/copper-containing carbon monoxide dehydrogenase, molybdoenzyme carbon monoxide dehydrogenase
ECTree
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Crystallization
Crystallization on EC 1.2.5.3 - aerobic carbon monoxide dehydrogenase
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enzyme in complex with inhibitor n-butylisonitrile, PDB ID 1N62, structure analysis
purified reconstituted enzyme, hangig drop vapour diffusion method, X-ray diffraction structure determination and analysis at 1.7 A resolution
vapor diffusion method using 0.8 M KH2PO4, 0.8 M NaH2PO4, 2% MPD, and 100 mM HEPES, pH 7.3, crystals containing cyanide are cocrystallized in the presence of 4 mM potassium cyanide, X-ray diffraction structure determination and analysis at 2.36-2.5 A resolution
crystallization at high (Moplus CODH)cand low intracellular molybdenum content (Mominus CODH), hanging drop vapour-diffusion method, Moplus CODH species at 2.5 units/mg obtained by mixing 0.006 ml of protein in 50 mM Hepes/NaOH, pH 7.2, with 0.002 ml of reservoir solution containing 1.1 M NaK-tartrate, 0.3 M (NH4)H2PO4, pH 7.2, 3% w/v methylpentanediol, and 10 mM dithioerythritol, 1-2 weeks, 4°C, from the Mominus CODH species (0.02 units/mg) under the same crystallization conditions only strong bunched crystals in a brown precipitate emerge. Crystals suitable for X-ray data collection are prepared by repeated washing of these crystals with crystallizing agent to remove precipitate, redissolving of crystals in 50 mM Hepes/NaOH followed by recrystallization under the above conditions, X-ray diffraction structure determination and analysis at 2.25 A and 2.35 A resolution, respectively