1.14.11.47: [50S ribosomal protein L16]-arginine 3-hydroxylase
This is an abbreviated version!
For detailed information about [50S ribosomal protein L16]-arginine 3-hydroxylase, go to the full flat file.
Reaction
Synonyms
50S ribosomal protein L16 arginine hydroxylase, ycfD
ECTree
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Substrates Products
Substrates Products on EC 1.14.11.47 - [50S ribosomal protein L16]-arginine 3-hydroxylase
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REACTION DIAGRAM
AVSEATIR + 2-oxoglutarate + O2
[AVSEATIR]-(3R)-3-hydroxy-L-Arg8 + succinate + CO2
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KPITEKPLAVRMGKGKGNVE + 2-oxoglutarate + O2
[KPITEKPLAVRMGKGKGNVE]-(3R)-3-hydroxy-L-Arg11 + succinate + CO2
peptide derived from 50S ribosomal protein L16
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RLLPAVSEATIRRL + 2-oxoglutarate + O2
[RLLPAVSEATIRRL]-(3R)-3-hydroxy-L-Arg12 + succinate + CO2
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modification occurs at the arginine at the -3 position relative to the C-terminus
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[50S ribosomal cyclic peptide 6KKPAEVRMGKGKG88-linker]-L-Arg82 + 2-oxoglutarate + O2
[50S ribosomal cyclic peptide 6KKPAEVRMGKGKG88-linker]-(3R)-3-hydroxy-L-Arg82 + succinate + CO2
[50S ribosomal peptide KPVTKKPAEVRMGKGKGSVE-NH2]-L-Arg + 2-oxoglutarate + O2
[50S ribosomal peptide KPVTKKPAEVRMGKGKGSVE-NH2]-(3R)-3-hydroxy-L-Arg + succinate + CO2
[50S ribosomal protein L16]-L-Arg81 + 2-oxoglutarate + O2
[50S ribosomal protein L16]-(3R)-3-hydroxy-L-Arg81 + succinate + CO2
[50S ribosomal protein L16]-L-Arg82 + 2-oxoglutarate + O2
[50S ribosomal protein L16]-(3R)-3-hydroxy-L-Arg82 + succinate + CO2
[50S ribosomal cyclic peptide 6KKPAEVRMGKGKG88-linker]-(3R)-3-hydroxy-L-Arg82 + succinate + CO2
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[50S ribosomal cyclic peptide 6KKPAEVRMGKGKG88-linker]-L-Arg82 + 2-oxoglutarate + O2
[50S ribosomal cyclic peptide 6KKPAEVRMGKGKG88-linker]-(3R)-3-hydroxy-L-Arg82 + succinate + CO2
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[50S ribosomal peptide KPVTKKPAEVRMGKGKGSVE-NH2]-(3R)-3-hydroxy-L-Arg + succinate + CO2
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[50S ribosomal peptide KPVTKKPAEVRMGKGKGSVE-NH2]-L-Arg + 2-oxoglutarate + O2
[50S ribosomal peptide KPVTKKPAEVRMGKGKGSVE-NH2]-(3R)-3-hydroxy-L-Arg + succinate + CO2
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[50S ribosomal protein L16]-(3R)-3-hydroxy-L-Arg81 + succinate + CO2
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[50S ribosomal protein L16]-L-Arg81 + 2-oxoglutarate + O2
[50S ribosomal protein L16]-(3R)-3-hydroxy-L-Arg81 + succinate + CO2
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more than 95% hydoxylation, residue Arg81 is the only residue observed to be hydroxylated
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[50S ribosomal protein L16]-(3R)-3-hydroxy-L-Arg82 + succinate + CO2
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[50S ribosomal protein L16]-L-Arg82 + 2-oxoglutarate + O2
[50S ribosomal protein L16]-(3R)-3-hydroxy-L-Arg82 + succinate + CO2
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binding of 2-oxoglutarate to isoform YcfD occurs in an exothermic manner, dissociation constant is 55.6 microM. Residues S116 and R140 are involved in binding
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additional information
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of the tested peptides, six or more residues are required for efficient hydroxylation. Hydroxylation is ablated when the C-terminus is extended to more closely resemble a protein. Hydroxylation occurs with incorporation of more than 90% 18O from 18O2 and occurs at C-3 of arginine leaing to a stereochemistry of the hydroxylated arginine of 2S,3R
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additional information
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enzyme displays a temperature-dependent variation in uL16 hydroxylation. Dioxygen availability can be a limiting factor for ycfD catalysis at high temperatures, consistent with incomplete uL16 hydroxylation observed
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additional information
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enzyme displays a temperature-dependent variation in uL16 hydroxylation. Dioxygen availability can be a limiting factor for ycfD catalysis at high temperatures, consistent with incomplete uL16 hydroxylation observed
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