1.13.12.9: phenylalanine 2-monooxygenase

This is an abbreviated version!
For detailed information about phenylalanine 2-monooxygenase, go to the full flat file.

Word Map on EC 1.13.12.9

1.13.12.9

fad

l-methionine

proenzyme

l-tyrosine

purple

ammonia

flavin

l-amino

noncatalytic

l-met

prosequence

charge-transfer

analysis

Reaction

Synonyms

L-Phe oxidase, L-phenylalanine oxidase, L-phenylalanine oxidase (deaminating and decarboxylating), oxidase, phenylalanine (deaminating, decarboxylating), PAOpt, proPAO

ECTree

1 Oxidoreductases

1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)

1.13.12 With incorporation of one atom of oxygen (internal monooxygenases or internal mixed-function oxidases)

1.13.12.9 phenylalanine 2-monooxygenase

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Results	in table
2	Activating Compound
13	AA Sequence
2	Application
3	CAS Registry Number
3	Cloned(Commentary)
5	Cofactor
3	Crystallization (Commentary)
14	Inhibitors
6	Ki Value [mM]
20	KM Value [mM]
2	Molecular Weight [Da]
8	Natural Substrates/ Products (Substrates)
78	Organism
2	Pathway
12	PDB ID
3	pH Optimum
1	pH Range
17	Protein Variants
4	Purification (Commentary)
3	Reaction
5	Reaction Type
20	Reference
1	Specific Activity [micromol/min/mg]
1	Storage Stability
61	Substrates and Products (Substrate)
4	Subunits
17	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
2	Temperature Range [°C]
3	Temperature Stability [°C]
2	Turnover Number [1/s]

Engineering

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Engineering on EC 1.13.12.9 - phenylalanine 2-monooxygenase

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K478A

Pseudomonas sp.

catalytic activity 1/200 of the wild-type enzyme

698757

M142A

Pseudomonas sp.

kcat is 1/10 of the wild-type enzyme, Km is elevated, affinity for oxygen seems decreased

R143A

R143K

Y536A

Y536F

K478A

Pseudomonas sp. P-501

catalytic activity 1/200 of the wild-type enzyme

M142A

Pseudomonas sp. P-501

kcat is 1/10 of the wild-type enzyme, Km is elevated, affinity for oxygen seems decreased

R143A

2 entries

R143K

2 entries

Y536F

Pseudomonas sp. P-501

mutant enzyme shows 17fold lower activity compared to wild-type. Wild-type PAOpt produces higher amounts of phenylacetamide (PAM) than phenylpyruvate (PPV) as expected, but PAM is not produced by the R143K and Y536F mutants