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0.26 - 1.6
1-(2-pyridyl)acetone
0.26 - 0.51
1-(2-thienyl)acetone
0.2 - 0.63
1-(3-pyridyl)acetone
4.3
1-(3-thienyl)acetone
-
pH 6.5, 50°C, mutant I86A
1.05 - 2.4
1-(4-pyridyl)acetone
1.5 - 8.1
1-phenylpropanone
0.23 - 0.62
2',4'-difluoro-acetophenone
0.018
2'-methylacetophenone
-
pH 6.5, 50°C, wild-type enzyme
50.1
2,3-Butanedione
-
pH 7.4, 30°C
0.65
3'-bromo-acetophenone
-
pH 6.5, 50°C, wild-type enzyme
0.41 - 1.3
3'-chloro-acetophenone
0.16
3'-iodo-acetophenone
-
pH 6.5, 50°C, wild-type enzyme
0.21
3'-methoxy-acetophenone
-
pH 6.5, 50°C, wild-type enzyme
0.18
3'-methylacetophenone
-
pH 6.5, 50°C, wild-type enzyme
0.025
3'-trifluoromethyl-acetophenone
-
pH 6.5, 50°C, wild-type enzyme
36.8 - 463
3,5-bis(trifluoromethyl)acetophenone
4.1
ethyl 4-chloro-3-oxobutanoate
-
pH 7.4, 30°C
0.26
1-(2-pyridyl)acetone
-
pH 6.5, 50°C, wild-type enzyme
1.6
1-(2-pyridyl)acetone
-
pH 6.5, 50°C, mutant I86A
0.26
1-(2-thienyl)acetone
-
pH 6.5, 50°C, wild-type enzyme
0.35
1-(2-thienyl)acetone
-
pH 6.5, 50°C, wild-type enzyme
0.51
1-(2-thienyl)acetone
-
pH 6.5, 50°C, mutant I86A
0.2
1-(3-pyridyl)acetone
-
pH 6.5, 50°C, mutant I86A
0.63
1-(3-pyridyl)acetone
-
pH 6.5, 50°C, wild-type enzyme
1.05
1-(4-pyridyl)acetone
-
pH 6.5, 50°C, mutant I86A
2.4
1-(4-pyridyl)acetone
-
pH 6.5, 50°C, wild-type enzyme
1.5
1-phenylpropanone
-
pH 6.5, 50°C, wild-type enzyme
8.1
1-phenylpropanone
-
pH 6.5, 50°C, mutant I86A
0.23
2',4'-difluoro-acetophenone
-
pH 6.5, 50°C, wild-type enzyme
0.62
2',4'-difluoro-acetophenone
-
pH 6.5, 50°C, mutant I86A
0.41
3'-chloro-acetophenone
-
pH 6.5, 50°C, mutant I86A
1.3
3'-chloro-acetophenone
-
pH 6.5, 50°C, wild-type enzyme
36.8
3,5-bis(trifluoromethyl)acetophenone
wild-type, pH 7.2, 30°C
277
3,5-bis(trifluoromethyl)acetophenone
mutant S154Y/L194I, pH 7.2, 30°C
463
3,5-bis(trifluoromethyl)acetophenone
mutant S154Y, pH 7.2, 30°C
1.08
acetone
-
pH 6.5, 50°C, wild-type enzyme
1.33
acetone
-
pH 6.5, 50°C, mutant I86A
1.9
acetophenone
-
pH 6.5, 50°C, wild-type enzyme
14.9
acetophenone
-
pH 6.5, 50°C, mutant I86A
75
NADH
-
wild-type, pH 6.5, 30°C
300
NADH
-
mutant R38P, pH 5.5, 30°C
944
NADH
wild-type, pH 7.2, 30°C
3846
NADH
mutant S154Y/L194I, pH 7.2, 30°C
4207
NADH
mutant S154Y, pH 7.2, 30°C
5100
NADPH
-
mutant R38P, pH 5.5, 30°C
9300
NADPH
-
wild-type, pH 6.5, 30°C