1.1.1.300: NADP-retinol dehydrogenase This is an abbreviated version! For detailed information about NADP-retinol dehydrogenase, go to the full flat file .
Reaction
retinol +
NADP+ =
retinal +
NADPH +
H+
Synonyms aldehyde dehydrogenase, ALDH, all-trans retinal reductase, all-trans-retinaldehyde reductase, all-trans-retinol dehydrogenase, CG2065, CG2070, CG30491, CG3842, DHRS3, EC 5.2.1.3, hRDH5, hRoDH, More, mouse RDH11, mRDH11, NADP(H)-dependent retinol dehydrogenase/reductase, NADP+-dependent all-trans-retinol dehydrogenase, NADPH-dependent retinal reductase, NDRD, NRDR, NRDRA1, NRDRB1, NRDRB2, peroxisomal NADP(H)-dependent retinol dehydrogenase-reductase, photoreceptor retinol dehydrogenase, photoreceptor-associated retinol dehydrogenase, photoreceptor-specific retinol dehydrogenase, prRDH, RalR1, RDH, RDH10, RDH11, Rdh11/12-like 1, Rdh11/12-like 2, Rdh11/12-like 3, RDH12, RDH13, RDH14, RDH8, retinal reductase, retinal reductase 1, retinaldehyde reductase, retinoid dehydrogenase/reductase, retinoid oxidoreductase complex, Retinol dehydrogenase, retinol dehydrogenase 10, retinol dehydrogenase 11, retinol dehydrogenase 12, retinol dehydrogenase 13, retinol dehydrogenase 8, retinol dehydrogenase [NADP+], retinol dehydrogenase-10, retinol dehydrogenases 11-like, retSDR1, RHD8, ROC, SDR16C4, short-chain dehydrogenase/reductase RRD
ECTree
KM Value
KM Value on EC 1.1.1.300 - NADP-retinol dehydrogenase
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0.0001
11-cis-retinal
pH 7.4, 37°C
0.0016
11-cis-retinol
pH 7.4, 37°C
0.62
13-cis-retinal
pH 7.4, 37°C, determined with NADP+
0.00014 - 0.19
9-cis-retinal
0.0016
9-cis-retinol
pH 7.4, 37°C
0.0032 - 0.0044
all-trans-3-hydroxyretinal
0.00006 - 0.5
all-trans-retinal
0.0006 - 1.3
all-trans-retinol
additional information
additional information
-
0.00014
9-cis-retinal
pH 7.4, 37°C
0.19
9-cis-retinal
pH 7.4, 37°C, determined with NADP+
0.0032
all-trans-3-hydroxyretinal
Km-value is determined for microsomal preparations expressing recombinant Drosophila proteins
0.0041
all-trans-3-hydroxyretinal
Km-value is determined for microsomal preparations expressing recombinant Drosophila proteins
0.0044
all-trans-3-hydroxyretinal
Km-value is determined for microsomal preparations expressing recombinant Drosophila proteins
0.00006
all-trans-retinal
-
wild-type
0.00012
all-trans-retinal
-
purified recombinant enzyme
0.0002
all-trans-retinal
-
membrane-bound wild-type
0.00027
all-trans-retinal
Km-value is determined for microsomal preparations expressing recombinant Drosophila proteins
0.00028
all-trans-retinal
-
mutant T49M
0.0004
all-trans-retinal
-
0.0004
all-trans-retinal
Km-value is determined for microsomal preparations expressing recombinant Drosophila proteins
0.00041
all-trans-retinal
-
mutant I51N
0.0006
all-trans-retinal
Km-value is determined for microsomal preparations expressing recombinant Drosophila proteins
0.0007
all-trans-retinal
Km-value is determined for microsomal preparations expressing recombinant Drosophila proteins
0.00126
all-trans-retinal
wild-type, pH 7.3, 37°C
0.0017
all-trans-retinal
-
mutant M146G, pH 7.3, 37°C
0.0023
all-trans-retinal
mutant M144G, pH 7.3, 37°C
0.003
all-trans-retinal
-
mutant M147G, pH 7.3, 37°C
0.0031
all-trans-retinal
-
wild-type isoform prRDH1, pH 7.3, 37°C
0.0032
all-trans-retinal
-
0.0044
all-trans-retinal
-
wild-type isoform prRDH1, pH 7.3, 37°C
0.3
all-trans-retinal
37°C
0.5
all-trans-retinal
pH 7.4, 37°C, determined with NADP+
0.0006
all-trans-retinol
-
purified recombinant enzyme
0.0007
all-trans-retinol
-
membrane-bound wild-type
0.004
all-trans-retinol
pH 7.4, 37°C
0.004
all-trans-retinol
Km-value for all-trans-retinol is similar to that for retinal however, the rate of retinol oxidation by RDH13 is extremely low
0.18
all-trans-retinol
37°C
1.3
all-trans-retinol
pH 7.4, 37°C, determined with NADPH
0.0096
estrone
mutant M144G, pH 7.3, 37°C
0.0233
estrone
-
mutant M147G, pH 7.3, 37°C
0.0307
estrone
-
mutant M146G, pH 7.3, 37°C
0.004
NAD+
-
at pH 7.0 and 37°C
680
NAD+
pH 7.4, 37°C, determined with all-trans-retinol
2.22
NADH
pH 7.4, 37°C
6
NADH
pH and temperature not specified in the publication. The apparent Km value for NADH is three orders of magnitude higher than that for NADPH
1300
NADH
pH 7.4, 37°C, determined with all-trans-retinal
0.0004
NADP+
-
membrane-bound wild-type
0.001
NADP+
-
purified recombinant enzyme
0.0012
NADP+
pH 7.4, 37°C
0.0032
NADP+
pH 7.4, 37°C
0.0042
NADP+
-
at pH 7.0 and 37°C
0.8
NADP+
pH 7.4, 37°C, determined with all-trans-retinol
0.00047
NADPH
-
purified recombinant enzyme
0.00048
NADPH
-
membrane-bound wild-type
0.00074
NADPH
pH 7.4, 37°C
0.0012
NADPH
pH 7.4, 37°C
0.033
NADPH
-
mutant T49M
0.04
NADPH
-
at pH 7.0 and 37°C
0.105
NADPH
-
mutant I51N
0.23
NADPH
pH 7.4, 37°C, determined with all-trans-retinal
0.007
retinal
-
with NAD+ as cosubstrate, at pH 7.0 and 37°C
0.13
retinal
-
with NADPH as cosubstrate, at pH 7.0 and 37°C
additional information
additional information
CG2070-expessing microsomes do not show saturation with up to 0.008 mM all-trans-3-hydroxyretinaldehyde
-
additional information
additional information
CG2070-expessing microsomes do not show saturation with up to 0.008 mM all-trans-3-hydroxyretinaldehyde
-
additional information
additional information
CG2070-expessing microsomes do not show saturation with up to 0.008 mM all-trans-3-hydroxyretinaldehyde
-
additional information
additional information
CG2070-expessing microsomes do not show saturation with up to 0.008 mM all-trans-3-hydroxyretinaldehyde
-
additional information
additional information
-
recombinant RRD reduces free retinal (not bound with cellular retinol binding protein) with a K0.5 value of 0.0023 mM and a Hill constant of 1.7, and reduces CRBP(I)-bound retinal (2fold molar excess of cellular retinol binding protein(I) at each retinal concentration) with a K0.5 value of 0.0086 mM and a Hill constant of 2.1
-