Sequence of NEP1_YEAST

EC Number
Recommended Name
Accession Code
Organism
No of amino acids
Molecular Weight [Da]
Source
rRNA small subunit pseudouridine methyltransferase Nep1
Q06287
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
252
27895
Reaction
S-adenosyl-L-methionine + pseudouridine1191 in yeast 18S rRNA = S-adenosyl-L-homocysteine + N1-methylpseudouridine1191 in yeast 18S rRNA
Sequences with same EC No.
Sequence
show sequence in fasta format
  0 MVEDSRVRDA LKGGDQKALP ASLVPQAPPV LTSKDKITKR MIVVLAMASL ETHKISSNGP
 60 GGDKYVLLNC DDHQGLLKKM GRDISEARPD ITHQCLLTLL DSPINKAGKL QVYIQTSRGI
120 LIEVNPTVRI PRTFKRFSGL MVQLLHKLSI RSVNSEEKLL KVIKNPITDH LPTKCRKVTL
180 SFDAPVIRVQ DYIEKLDDDE SICVFVGAMA RGKDNFADEY VDEKVGLSNY PLSASVACSK
240 FCHGAEDAWN IL
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Sequence related references
Sequence Reference
Authors
Title
Journal
Volume
Pages
Year
PubMed ID
186445
Johnston M.,Hillier L.W.,Riles L.,Albermann K.,Andre B.,Ansorge W.,Benes V.,Brueckner M.,Delius H.,Dubois E.,Duesterhoeft A.,Entian K.-D.,Floeth M.,Goffeau A.,Hebling U.,Heumann K.,Heuss-Neitzel D.,Hilbert H.,Hilger F.,Kleine K.,Koetter P.,Louis E.J.,Messenguy F.,Mewes H.-W.,Miosga T.,Moestl D.,Mueller-Auer S.,Nentwich U.,Obermaier B.,Piravandi E.,Pohl T.M.,Portetelle D.,Purnelle B.,Rechmann S.,Rieger M.,Rinke M.,Rose M.,Scharfe M.,Scherens B.,Scholler P.,Schwager C.,Schwarz S.,Underwood A.P.,Urrestarazu L.A.,Vandenbol M.,Verhasselt P.,Vierendeels F.,Voet M.,Volckaert G.,Voss H.,Wambutt R.,Wedler E.,Wedler H.,Zimmermann F.K.,Zollner A.,Hani J.,Hoheisel J.D.
The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
Nature
387
87-90
1997
186446
Engel S.R.,Dietrich F.S.,Fisk D.G.,Binkley G.,Balakrishnan R.,Costanzo M.C.,Dwight S.S.,Hitz B.C.,Karra K.,Nash R.S.,Weng S.,Wong E.D.,Lloyd P.,Skrzypek M.S.,Miyasato S.R.,Simison M.,Cherry J.M.
The reference genome sequence of Saccharomyces cerevisiae: Then and now.
G3 (Bethesda)
4
389-398
2014
186447
Hu Y.,Rolfs A.,Bhullar B.,Murthy T.V.S.,Zhu C.,Berger M.F.,Camargo A.A.,Kelley F.,McCarron S.,Jepson D.,Richardson A.,Raphael J.,Moreira D.,Taycher E.,Zuo D.,Mohr S.,Kane M.F.,Williamson J.,Simpson A.J.G.,Bulyk M.L.,Harlow E.,Marsischky G.,Kolodner R.D.,LaBaer J.
Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae.
Genome Res.
17
536-543
2007
186448
Liu P.C.,Thiele D.J.
Novel stress-responsive genes EMG1 and NOP14 encode conserved, interacting proteins required for 40S ribosome biogenesis.
Mol. Biol. Cell
12
3644-3657
2001
186449
Eschrich D.,Buchhaupt M.,Koetter P.,Entian K.-D.
Nep1p (Emg1p), a novel protein conserved in eukaryotes and archaea, is involved in ribosome biogenesis.
Curr. Genet.
40
326-338
2002
186450
Bernstein K.A.,Gallagher J.E.G.,Mitchell B.M.,Granneman S.,Baserga S.J.
The small-subunit processome is a ribosome assembly intermediate.
Eukaryot. Cell
3
1619-1626
2004
186451
Taylor A.B.,Meyer B.,Leal B.Z.,Kotter P.,Schirf V.,Demeler B.,Hart P.J.,Entian K.D.,Wohnert J.
The crystal structure of Nep1 reveals an extended SPOUT-class methyltransferase fold and a pre-organized SAM-binding site.
Nucleic Acids Res.
36
1542-1554
2008
186452
Meyer B.,Wurm J.P.,Kotter P.,Leisegang M.S.,Schilling V.,Buchhaupt M.,Held M.,Bahr U.,Karas M.,Heckel A.,Bohnsack M.T.,Wohnert J.,Entian K.D.
The Bowen-Conradi syndrome protein Nep1 (Emg1) has a dual role in eukaryotic ribosome biogenesis, as an essential assembly factor and in the methylation of Psi1191 in yeast 18S rRNA.
Nucleic Acids Res.
39
1526-1537
2011
186453
Leulliot N.,Bohnsack M.T.,Graille M.,Tollervey D.,Van Tilbeurgh H.
The yeast ribosome synthesis factor Emg1 is a novel member of the superfamily of alpha/beta knot fold methyltransferases.
Nucleic Acids Res.
36
629-639
2008
186454
Thomas S.R.,Keller C.A.,Szyk A.,Cannon J.R.,Laronde-Leblanc N.A.
Structural insight into the functional mechanism of Nep1/Emg1 N1-specific pseudouridine methyltransferase in ribosome biogenesis.
Nucleic Acids Res.
39
2445-2457
2011